Component | Usage | Quantity | Catalog # |
Anti-Bovine CCL2 (MCP-1) Polyclonal Antibody | Capture Antibody | 100 µg | PB0092B-100 |
Biotinylated Anti-Bovine CCL2 (MCP-1) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0091B-050 |
Bovine CCL2 (MCP-1) Recombinant Protein | Standard | 5 µg | RP0027B-005 |
The Bovine CCL2 (MCP-1) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CCL2 (MCP-1) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine CCL2 (MCP-1) Do-It-Yourself ELISA can also be used to measure Wild Yak CCL2.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemotactic protein-1 (MCP-1), is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CCL2 (MCP-1) recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. CCL2 (MCP-1) is implicated in pathogeneses of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis.
Alternate Names - CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF, C-C motif chemokine ligand 2
The chemokines CCL2 and CXCL10 produced by bovine endometrial epithelial cells induce migration of bovine B lymphocytes, contributing to transuterine transmission of BLV infection.
Andoh K, Nishimori A, Sakumoto R, Hayashi KG, Hatama S.
Vet Microbiol. 2020 Mar;242:108598. doi: 10.1016/j.vetmic.2020.108598. Epub 2020 Jan 28.
Applications: Measurement of bovine CXCL10 and CCL2 in supernatant of BEnEpCs by ELISA
Maternal intravenous administration of azithromycin results in significant fetal uptake in a sheep model of second trimester pregnancy.
Kemp MW, Miura Y, Payne MS, Jobe AH, Kallapur SG, Saito M, Stock SJ, Spiller OB, Ireland DJ, Yaegashi N, Clarke M, Hahne D, Rodger J, Keelan JA, Newnham JP.
Antimicrob Agents Chemother. 2014 Nov;58(11):6581-91. doi: 10.1128/AAC.03721-14. Epub 2014 Aug 25.
Applications: Measurement by ELISA of MCP-2 (CCL2) in sheep amniotic fluid.
Maternal intravenous treatment with either azithromycin or solithromycin clears Ureaplasma parvum from the amniotic fluid in an ovine model of intrauterine infection.
Miura Y, Payne MS, Keelan JA, Noe A, Carter S, Watts R, Spiller OB, Jobe AH, Kallapur SG, Saito M, Stock SJ, Newnham JP, Kemp MW.
Antimicrob Agents Chemother. 2014 Sep;58(9):5413-20. doi: 10.1128/AAC.03187-14. Epub 2014 Jun 30.
Applications: Measurement by ELISA of MCP-2 (CCL2) in sheep amniotic fluid.
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