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CartBovine CXCL9 (MIG) Do-It-Yourself ELISA, ≤10 Plates
Bovine CXCL9 ELISA Data
Bovine CXCL9 ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Bovine CXCL9 (MIG) Polyclonal Antibody | Capture Antibody | 100 µg | PB0122B-100 |
| Biotinylated Anti-Bovine CXCL9 (MIG) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0265B-050 |
| Bovine CXCL9 (MIG) Recombinant Protein | Standard | 5 µg | RP0025B-005 |
Bovine CXCL9 ELISA Specifications
The Bovine CXCL9 (MIG) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CXCL9 (MIG) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine CXCL9 (MIG) Do-It-Yourself ELISA can also be used to measure Bison, Wild Yak, and Zebu CXCL9 (MIG).
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
CXCL9 Background
Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family. It is also commonly known as Monokine Induced by Gamma interferon (MIG). CXCL9/MIG is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines -CXCL10 and CXCL11. There have been 17 different C-X-C chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the C-X-C motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive C-X-C chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. C-X-C chemokines that lack the ELR motif are chemoattractant for lymphocytes. CXCL9 (MIG) elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Alternate Names - CXCL9, CMK, Humig, MIG, SCYB9, crg-10, C-X-C motif chemokine ligand 9
Biomarkers of cell-mediated immunity to bovine tuberculosis.
Palmer MV, Thacker TC, Rabideau MM, Jones GJ, Kanipe C, Vordermeier HM, Ray Waters W.
Vet Immunol Immunopathol. 2020 Feb;220:109988. doi: 10.1016/j.vetimm.2019.109988. Epub 2019 Nov 30.
Applications: Measurement of bovine CXCL9 and CXCL10 in plasma by ELISA
Abstract
Whole blood based assays, particularly interferon gamma (IFN-γ) release assays (IGRAs), are used for the diagnosis of both bovine and human tuberculosis (TB). The aim of the current study was to evaluate a panel of cytokines and chemokines for potential use as diagnostic readouts indicative of Mycobacterium bovis (M. bovis) infection in cattle. A gene expression assay was used to determine the kinetics of the response to M. bovis purified protein derivative and a fusion protein consisting of ESAT-6, CFP10, and Rv3615c upon aerosol infection with ∼104 cfu of M. bovis. The panel of biomarkers included: IFN-γ, CXCL9, CXCL10, CCL2, CCL3, TNF-α, IL-1α, IL-1β, IL-1Ra, IL-22, IL-21 and IL-13. Protein levels of IFN-γ, CXCL9, and CXCL10 were determined by ELISA. Findings suggest that CXCL9, CXCL10, IL-21, IL-13, and several acute phase cytokines may be worth pursuing as diagnostic biomarkers of M. bovis infection in cattle.
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