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CartBovine CXCL10 (IP-10) Do-It-Yourself ELISA, ≤10 Plates
Bovine CXCL10 ELISA Data
Bovine CXCL10 ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Bovine CXCL10 (IP-10) Polyclonal Antibody | Capture Antibody | 100 µg | PB0385B-100 |
| Biotinylated Anti-Bovine CXCL10 (IP-10) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0393B-050 |
| Bovine CXCL10 (IP-10) Recombinant Protein | Standard | 5 µg | RP0079B-005 |
Specifications
The Bovine CXCL10 (IP-10) Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Bovine CXCL10 (IP-10) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine CXCL10 (IP-10) Do-It-Yourself ELISA can also be used to measure Bison, and Wild Yak CXCL10 (IP-10).
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Background
C-X-C motif chemokine 10 (CXCL10) also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 is a member of the C-X-C chemokine family. CXCL10 (IP-10) is secreted by several cell types in response to IFN-gamma. These cell types include monocytes, endothelial cells and fibroblasts. CXCL10 (IP-10) has been attributed to several roles, such as chemoattraction for monocytes/macrophages, T cells, NK cells, and dendritic cells, promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation and angiogenesis. There have been 17 different C-X-C chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the C-X-C motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive C-X-C chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. C-X-C chemokines that lack the ELR motif are chemoattractant for lymphocytes. CXCL10 (IP-10) elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Alternate Names - CXCL10, C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1, C-X-C motif chemokine ligand 10, C-X-C motif chemokine 10
The chemokines CCL2 and CXCL10 produced by bovine endometrial epithelial cells induce migration of bovine B lymphocytes, contributing to transuterine transmission of BLV infection.
Andoh K, Nishimori A, Sakumoto R, Hayashi KG, Hatama S.
Vet Microbiol. 2020 Mar;242:108598. doi: 10.1016/j.vetmic.2020.108598. Epub 2020 Jan 28.
Applications: Measurement of bovine CXCL10 and CCL2 in supernatant of BEnEpCs by ELISA
Biomarkers of cell-mediated immunity to bovine tuberculosis.
Palmer MV, Thacker TC, Rabideau MM, Jones GJ, Kanipe C, Vordermeier HM, Ray Waters W.
Vet Immunol Immunopathol. 2020 Feb;220:109988. doi: 10.1016/j.vetimm.2019.109988. Epub 2019 Nov 30.
Applications: Measurement of bovine CXCL9 and CXCL10 in plasma by ELISA
Abstract
Whole blood based assays, particularly interferon gamma (IFN-γ) release assays (IGRAs), are used for the diagnosis of both bovine and human tuberculosis (TB). The aim of the current study was to evaluate a panel of cytokines and chemokines for potential use as diagnostic readouts indicative of Mycobacterium bovis (M. bovis) infection in cattle. A gene expression assay was used to determine the kinetics of the response to M. bovis purified protein derivative and a fusion protein consisting of ESAT-6, CFP10, and Rv3615c upon aerosol infection with ∼104 cfu of M. bovis. The panel of biomarkers included: IFN-γ, CXCL9, CXCL10, CCL2, CCL3, TNF-α, IL-1α, IL-1β, IL-1Ra, IL-22, IL-21 and IL-13. Protein levels of IFN-γ, CXCL9, and CXCL10 were determined by ELISA. Findings suggest that CXCL9, CXCL10, IL-21, IL-13, and several acute phase cytokines may be worth pursuing as diagnostic biomarkers of M. bovis infection in cattle.
Impact of Mycobacterium bovis-induced pathology on interpretation of QuantiFERON®-TB Gold assay results in African buffaloes (Syncerus caffer).
Bernitz N, Kerr TJ, Goosen WJ, Higgitt RL, de Waal C, Clarke C, Cooper DV, Warren RM, van Helden PD, Parsons SDC, Miller MA.
Vet Immunol Immunopathol. 2019 Nov;217:109923. doi: 10.1016/j.vetimm.2019.109923. Epub 2019 Aug 8.
Applications: Measurement of African Buffalo CXCL10 in plasma by ELISA
Interleukin 8 and Pentaxin (C-Reactive Protein) as Potential New Biomarkers of Bovine Tuberculosis.
Gao X, Guo X, Li M, Jia H, Lin W, Fang L, Jiang Y, Zhu H, Zhang Z, Ding J, Xin T.
J Clin Microbiol. 2019 Sep 24;57(10). pii: e00274-19. doi: 10.1128/JCM.00274-19. Print 2019 Oct.
Applications: Measurement of bovine IL-17A and CXCL10 (IP-10) in serum and plasma by ELISA
Parallel measurement of IFN-γ and IP-10 in QuantiFERON®-TB Gold (QFT) plasma improves the detection of Mycobacterium bovis infection in African buffaloes (Syncerus caffer).
Bernitz N, Kerr TJ, Goosen WJ, Clarke C, Higgitt R, Roos EO, Cooper DV, Warren RM, van Helden PD, Parsons SDC, Miller MA.
Prev Vet Med. 2019 Aug 1;169:104700. doi: 10.1016/j.prevetmed.2019.104700. Epub 2019 May 23.
Applications: Measurement of African Buffaloes CXCL10 in plasma by ELISA
Antigen-Specific IP-10 Release Is a Sensitive Biomarker of Mycobacterium bovis Infection in Cattle
Parsons SD, McGill K, Doyle MB, Goosen WJ, van Helden PD, Gormley E.
PLoS One. 2016 May 11;11(5):e0155440. doi: 10.1371/journal.pone.0155440. eCollection 2016.
Applications: Measurement of CXCL10 in plasma of cattle by ELISA
Abstract
The most widely used ante-mortem diagnostic tests for tuberculosis in cattle are the tuberculin skin test and the interferon-gamma (IFN-γ) release assay, both of which measure cell-mediated immune responses to Mycobacterium bovis infection. However, limitations in the performance of these tests results in a failure to identify all infected animals. In attempting to increase the range of diagnostic tests for tuberculosis, measurement of the cytokine IP-10 in antigen-stimulated blood has previously been shown to improve the detection of M. tuberculosis and M. bovis infection, in humans and African buffaloes (Syncerus caffer), respectively. In the present study, 60 cattle were identified by the single intradermal comparative tuberculin test as tuberculosis reactors (n = 24) or non-reactors (n = 36) and the release of IFN-γ and IP-10 in antigen-stimulated whole blood from these animals was measured using bovine specific ELISAs. There was a strong correlation between IP-10 and IFN-γ production in these samples. Moreover, measurement of the differential release of IP-10 in response to stimulation with M. bovis purified protein derivative (PPD) and M. avium PPD distinguished between reactor and non-reactor cattle with a sensitivity of 100% (95% CI, 86%-100%) and a specificity of 97% (95% CI, 85%-100%). These results suggest that IP-10 might prove valuable as a diagnostic biomarker of M. bovis infection in cattle.
IP-10 Is a Sensitive Biomarker of Antigen Recognition in Whole-Blood Stimulation Assays Used for the Diagnosis of Mycobacterium bovis Infection in African Buffaloes (Syncerus caffer).
Goosen WJ, Cooper D, Miller MA, van Helden PD, Parsons SD.
Clin Vaccine Immunol. 2015 Aug;22(8):974-8. doi: 10.1128/CVI.00324-15. Epub 2015 Jun 24.
Applications: Measurement of CXCL10 in plasma samples of African buffaloes by ELISA
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