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CartBovine IFN alpha A Do-It-Yourself ELISA, ≤10 Plates
Bovine IFNα A ELISA Data
Bovine IFNα A ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Bovine IFNαA Polyclonal Antibody | Capture Antibody | 100 µg | KP1840B-100 |
| Biotinylated Anti-Bovine IFNαA Polyclonal Antibody | Detection Antibody | 50 µg | KPB1841B-050 |
| Bovine IFNαA Recombinant Protein | Standard | 5 µg | RP0008B-005 |
Bovine IFNα A ELISA Specifications
The Bovine IFNαA Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine IFNαA ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IFNα Background
Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-α, IFN-β, IFN-κ, IFN-δ, IFN-ε, IFN-τ, IFN-ω, and IFN-ζ (also known as limitin). They are mainly involved in innate immune response against viral infection.
A TLR7 agonist activates bovine Th1 response and exerts antiviral activity against bovine leukemia virus.
Sajiki Y, Konnai S, Okagawa T, Maekawa N, Nakamura H, Kato Y, Suzuki Y, Murata S, Ohashi K.
Dev Comp Immunol. 2020 Sep 2;114:103847. doi: 10.1016/j.dci.2020.103847.
Applications: Measurement of bovine TNF alpha, IFN alpha, and IFN gamma in culture supernatants by ELISA
Increased Susceptibility of Cattle to Intranasal RVFV Infection.
Kroeker AL, Smid V, Embury-Hyatt C, Collignon B, Pinette M, Babiuk S, Pickering B.
Front Vet Sci. 2020 Apr 29;7:137. doi: 10.3389/fvets.2020.00137. eCollection 2020.
Applications: Measurement of bovine IFN alpha and IFN beta in nasal and oral swabs by ELISA.
Abstract
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