Component | Use | Quantity | Catalog # |
Anti-Bovine IL-1 Receptor Antagonist Polyclonal Antibody | Capture Antibody | 100 µg | PB0476B-100 |
Biotinylated Anti-Bovine IL-1 Receptor Antagonist Polyclonal Antibody | Detection Antibody | 50 µg | PBB0485B-050 |
Bovine IL-1RA Recombinant Protein | Standard | 5 µg | RP0351B-005 |
The Bovine IL-1 Receptor Antagonist (IL-1RA) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine IL-1RA ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine IL-1RA Do-It-Yourself ELISA can also be used to measure Bison, Water Buffalo, Wild Yak, and Zebu IL-1RA
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Alternate Names - IL1RN, DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4, interleukin 1 receptor antagonist
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