Component | Use | Quantity | Catalog # |
Anti-Bovine IL-15 Polyclonal Antibody | Capture Antibody | 100 µg | PB0447B-100 |
Biotinylated Anti-Bovine IL-15 Polyclonal Antibody | Detection Antibody | 50 µg | PBB0453B-050 |
Bovine IL-15 Recombinant Protein | Standard | 5 µg | RP0009B-005 |
The Bovine IL-15 Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Bovine IL-15 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine IL-15 Do-It-Yourself ELISA can also be used to measure Water Buffalo, Wild Yak, and Zebu IL-15.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Interleukin 15 (IL-15) is a cytokine with structural similarity to IL-2 that is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces cell proliferation of natural killer cells; cells of the innate immune system whose principal role is to kill virally infected cells. IL-15 was discovered to be a member of a family of cytokines, which also includes IL-2, IL-4, IL-7, IL-9, and IL-21. The specific receptor for IL-15, IL-15 receptor alpha, appears to regulate IL-15 secretion, stability, and activity. All members of this family use the common gamma chain as part of their signaling complex. IL-15 has been shown to play a role in many diseases, including obesity, influenza, and inflammatory autoimmune diseases.
Alternate Names - IL15, IL-15, interleukin 15
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