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CartBovine IL-21 Do-It-Yourself ELISA, ≤10 Plates
Bovine IL-21 ELISA Data
Bovine IL-21 ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Bovine IL-21 Polyclonal Antibody | Capture Antibody | 100 µg | PB0325B-100 |
| Biotinylated Anti-Bovine IL-21 Polyclonal Antibody | Detection Antibody | 50 µg | PBB0326B-050 |
| Bovine IL-21 Recombinant Protein | Standard | 5 µg | RP0074B-005 |
Bovine IL-21 ELISA Specifications
The Bovine IL-21 Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Bovine IL-21 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-21 Background
IL-21 is a member of the type I cytokine family, which includes IL-2 and IL-15. IL-21 signals through the common gamma-chain and a unique IL-21 receptor. The IL-21 receptor is expressed in cells lymphoid-lineage and regulates the proliferation and differentiation of T and B lymphocytes. Studies suggest that IL-21 is a key component of CD4 T cell help that is required for maintaining the CD8+ T cell responses and inducing B cell antibody response. IL-21 also promotes the development of Th17 and Tfh cells, modulates the cytotoxic activity and survival of NK and CD8+ T cells, and suppresses the maturation of dendritic cells. It is also implicated in the development of autoimmune disease and has antitumor activity.
Alternate Names - IL21, CVID11, IL-21, Za11, interleukin 21
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