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CartBovine VEGF-A Do-It-Yourself ELISA, ≤10 Plates
Bovine VEGF-A ELISA Data
Bovine VEGF-A ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Bovine VEGF-A Polyclonal Antibody | Capture Antibody | 100 µg | PB0276B-100 |
| Biotinylated Anti-Bovine VEGF-A Polyclonal Antibody | Detection Antibody | 50 µg | PBB0279B-050 |
| Bovine VEGF-A Recombinant Protein | Standard | 5 µg | RP0072B-005 |
Specifications
The Bovine VEGF-A Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Bovine VEGF-A ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine VEGF-A Do-It-Yourself ELISA can also be used to measure Bison, and Water Buffalo VEGF-A.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Background
Vascular endothelial growth factor (VEGF) proteins stimulate vasculogenesis and angiogenesis. They are part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. The normal function of VEGF proteins is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. The VEGF family has six members, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placental Growth Factor (PGF). Activity of VEGF-A, as its name implies, has been studied mostly on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor (VPF).
Alternate Names - VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A
Effect of ovarian steroids on vascular endothelial growth factor a expression in bovine uterine endothelial cells during adenomyosis.
Lupicka M, Zadroga A, Szczepańska A, Korzekwa AJ.
BMC Vet Res. 2019 Dec 30;15(1):473. doi: 10.1186/s12917-019-2222-0.
Applications: Measurement of bovine VEGF-A in culture media by ELISA
Abstract
BACKGROUND:
Adenomyosis is a uterine dysfunction defined as the presence of endometrial glands within the myometrium. There is evidence that proangiogenic factors may play a role during the development of adenomyosis; however, exact mechanism remains unknown. The aim of the study was to determine the action of vascular endothelial growth factor A (VEGFA) in uterine tissue and uterine vascular endothelial cells during adenomyosis.
RESULTS:
Uterine tissues were collected and examined for the presence and extent of adenomyosis. Gene and protein expression of VEGFA and its two receptors (VEGFR1 and VEGFR2) was evaluated with quantitative polymerase chain reaction and Western blotting, respectively, in endometrium and myometrium during adenomyosis. Immunolocalization of VEGFA and its receptors within uterine tissues during adenomyosis was also determined. In an in vitro experiment, endothelial cells from non-adenomyotic bovine uteri were treated with media conditioned by non-adenomyotic or adenomyotic uterine slices treated with 17-beta-oestradiol (E2) or progesterone (P4). Both gene and protein expression of VEGFR2 were elevated in endometrium in stages 3-4 of adenomyosis. Protein expression of VEGFA and VEGFR2 as well as VEGFA secretion were increased in endothelial cells treated with media conditioned by adenomyotic uterine slices after E2 treatment.
CONCLUSIONS:
Results suggest that VEGFA signalling is an important component, next to E2, that enhances VEGFA action and participates in adenomyosis development in cows.
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