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CartChicken IL-16 Do-It-Yourself ELISA, ≤10 Plates
Chicken IL-16 ELISA Data
Chicken IL-16 ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Chicken IL-16 Polyclonal Antibody | Capture Antibody | 100 µg | KP1155C-100 |
| Biotinylated Anti-Chicken IL-16 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1156C-050 |
| Chicken IL-16 Recombinant Protein Standard | Standard | 5 µg | RP0019C-005 |
Chicken IL-16 ELISA Specifications
The Chicken IL-16 Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Chicken IL-16 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-16 Background
Interleukin 16 (IL-16) is a pleiotropic cytokine that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4, and has effects on mixed lymphocyte reaction and inhibition of HIV viral replication. Because this cytokine was discovered in 1982 for its T lymphocyte chemotactic activity, it was named “Lymphocyte Chemoattractant Factor (LCF)” until it was designated interleukin-16. IL-16 forms homotetramers and this structure is required for its bioactivity. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. IL-16 is released by a variety of immune (T cells, esinophils, and dendritic cells) and non-immune (fibroblasts, epithelial, and neurona) cells. In the non-diseased state, IL-16 mRNA is almost exclusively expressed on lymphatic tissue, and high levels in T cells. During inflammation, IL-16 is synthesized in a number of other tissues. The sequence, structure, and function of IL-16 are highly conserved in all species examined. Thus far, all species of IL-16 tested induce similar bioactivities on CD4+ T cells.
Alternate Names - IL16, LCF, NPRprIL-16, IL-16, interleukin 16
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