Component | Usage | Quantity | Catalog # |
Anti-Equine IL-2 Polyclonal Antibody | Capture Antibody | 100 µg | PB0050E-100 |
Biotinylated Anti-Equine IL-2 Polyclonal Antibody | Detection Antibody | 50 µg | PBB0051E-050 |
Equine IL-2 Recombinant Protein | Standard | 5 µg | RP0006E-005 |
The Equine IL-2 Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Equine IL-2 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Interleukin-2 (IL-2) is a cytokine produced by T-helper cells in response to antigenic or mitogenic stimulation. It is required for T-cell proliferation and other activities crucial to the regulation of the immune response. IL-2 was discovered to be a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and a common gamma chain (γc). All members of this family use the common gamma chain as part of their signaling complex.
Alternate Names - IL2, IL-2, TCGF, lymphokine, interleukin 2
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