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CartMouse IL-13 Do-It-Yourself ELISA, ≤10 Plates
Mouse IL-13 ELISA Data
Mouse IL-13 ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Mouse IL-13 Polyclonal Antibody | Capture Antibody | 100 µg | KP1215M-100 |
| Biotinylated Anti-Mouse IL-13 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1216M-050 |
| Mouse IL-13 Recombinant Protein | Standard | 5 µg | RP0735M-005 |
Mouse IL-13 ELISA Specifications
The Mouse IL-13 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Mouse IL-13 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-13 Background
Interleukin 13 (IL-13) is secreted by many cell types, but especially T helper type 2 (Th2) cells. IL-13 is an important mediator of allergic inflammation and disease. In addition to effects on immune cells, IL-13 is implicated as a central mediator of the physiologic changes induced by allergic inflammation in many tissues. The functions of IL-13 overlap considerably with those of IL-4, especially with regard to changes induced on hematopoietic cells, but these effects are probably less important given the more potent role of IL-4. Thus, although IL-13 can induce immunoglobulin E (IgE) secretion from activated human B cells, deletion of IL-13 from mice does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. In comparison, deletion of IL-4 abrogates these responses. Thus, rather than a lymphoid cytokine, IL-13 acts more prominently as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thereby altering physiological function.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Alternate Names - IL13, IL-13, P600, interleukin 13
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