Component | Use | Quantity | Catalog # |
Anti-Cynomolgus Monkey CXCL9 (MIG) Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1501Y-100 |
Biotinylated Anti-Cynomolgus Monkey CXCL9 (MIG) Polyclonal Antibody | Detection Antibody | 50 µg | KPB1502Y-050 |
Cynomolgus Monkey CXCL9 (MIG) Recombinant Protein | Standard | 5 µg | RP1340Y-005 |
The Cynomolgus Monkey CXCL9 (MIG) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Cynomolgus Monkey CXCL9 (MIG) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Chemokine (C-X-C motif) ligand 9 (CXCL9) is a small cytokine belonging to the CXC chemokine family. It is also commonly known as Monokine Induced by Gamma interferon (MIG). CXCL9/MIG is a T-cell chemoattractant, which is induced by IFN-gamma. It is closely related to two other CXC chemokines -CXCL10 and CXCL11. There have been 17 different C-X-C chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the C-X-C motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive C-X-C chemokines specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2. C-X-C chemokines that lack the ELR motif are chemoattractant for lymphocytes. CXCL9 (MIG) elicits its effects by binding to the cell surface chemokine receptor CXCR3.
Alternate Names - CXCL9, CMK, Humig, MIG, SCYB9, crg-10, C-X-C motif chemokine ligand 9
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