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CartCanine SCF Do-It-Yourself ELISA, ≤10 Plates
Canine SCF ELISA Data
Canine SCF ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Canine SCF Polyclonal Antibody | Capture Antibody | 100 µg | KP1563D-100 |
| Biotinylated Anti-Canine SCF Polyclonal Antibody | Detection Antibody | 50 µg | KPB1564D-050 |
| Canine SCF Recombinant Protein | Standard | 5 µg | RP1326D-005 |
Canine SCF ELISA Specifications
The Canine SCF Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Canine SCF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Canine SCF Do-It-Yourself ELISA can also be used to measure Dingo and Red Fox SCF.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
SCF Background
SCF (stem cell factor), often known as Kit Ligand, KITL or KITLG, is involved in pathways that control the regulation of cell survival, migration and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, and melanogenesis. SCF binding to the receptor-type protein-tyrosine kinase KIT, induces a cell signaling pathway that activates several other cell signaling pathways, including PIK3R1/AKT1, GRB2, RAS, RAF1, MAPK1/ERK2, MAPK3/ERK1, and STAT signaling events. The effects of SCF on these secondary pathways further allow it to play a role in many or the major cellular processes. The SCF, of most vertebrates, has a soluble form as well as the membrane bound type I receptor form. Mutations in the SCF pathway may result in a broad range of abnormalities including anemia, and sterility. SCF and KIT interaction has been shown to promote the growth of cancer cells and organoids in culture, and xenograft tumors in mice. SCF is being utilized in many animal model systems, from metastasis research in breast tumor-bearing arthritic mice to the genetic linkage to de-pigmentation in pigs. Interestingly, a second SCF pathway was retained in zebrafish, after a whole genome duplication, and is now undergoing divergent evolution.
Alternate Names - KITLG, FPH2, FPHH, KL-1, Kitl, MGF, SCF, SF, SHEP7, DCUA, KIT ligand, DFNA69, SLF
Ordering Information & Terms and Conditions
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Kingfisher Biotech, Inc.
1000 Westgate Drive
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USA
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Returns
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