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CartBovine Erythropoietin (EPO) Do-It-Yourself ELISA, ≤20 Plates
Bovine EPO ELISA Data
Bovine EPO ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Bovine Erythropoietin Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1583B-100 |
| Biotinylated Anti-Bovine Erythropoietin Polyclonal Antibody | Detection Antibody | 50 µg | KPB1584B-050 |
| Bovine Erythropoietin Recombinant Protein | Standard | 5 µg | RP1275B-005 |
Bovine EPO ELISA Specifications
The Bovine Erythropoietin Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine EPO ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine Erythropoietin Do-It-Yourself ELISA can also be used to measure Bison, Yak, and Zebu Erythropoietin (EPO).
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
EPO Background
Erythropoietin (EPO) is an essential hormone for red blood cell production. Without it, definitive erythropoiesis does not take place. Erythropoietin has its primary effect on red blood cell progenitors and precursors by promoting their survival through protecting these cells from apoptosis. It is the primary erythropoietic factor that cooperates with various other growth factors (e.g., IL-3, IL-6, glucocorticoids, and SCF) involved in the development of erythroid lineage from multipotent progenitors. Erythropoietin has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It can increase iron absorption by suppressing the hormone hepcidin. EPO is highly glycosylated, with half-life in blood around five hours. The half-life of EPO may vary between endogenous and various recombinant versions. Additional glycosylation or other alterations of EPO via recombinant technology have led to increased stability in blood. EPO binds to the erythropoietin receptor on the red cell progenitor surface and activates a JAK2 signaling cascade. Erythropoietin receptor expression is found in a number of tissues, such as bone marrow and peripheral/central nervous tissue. In the bloodstream, red cells themselves do not express erythropoietin receptor, so cannot respond to EPO. However, indirect dependence of red cell longevity in the blood on plasma erythropoietin levels has been reported, a process termed neocytolysis.
Alternate Names - EPO, EP, MVCD2, erythropoietin, Erythropoietin, ECYT5, DBAL
Ordering Information & Terms and Conditions
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Kingfisher Biotech, Inc.
1000 Westgate Drive
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Saint Paul, MN 55114
USA
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