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CartSwine IL-17A Polyclonal Antibody
Reactivity - ELISA
Bovine IL-17A - Moderate
Canine IL-17A - Weak
Equine IL-17A - Weak
Human IL-17A - Weak
Mouse IL-17A - None
Ovine IL-17A - Moderate
Rabbit IL-17A - Weak
Rat IL-17A - Weak
Swine IL-17A - Strong
Swine IL-17A ELISA Data
Deoxynivalenol as a new factor in the persistence of intestinal inflammatory diseases: an emerging hypothesis through possible modulation of Th17-mediated response.
Cano PM, Seeboth J, Meurens F, Cognie J, Abrami R, Oswald IP, Guzylack-Piriou L.
PLoS One. 2013;8(1):e53647. doi: 10.1371/journal.pone.0053647. Epub 2013 Jan 10.
Applications: Measurement of swine IL-17A in cell culture supernatants by ELISA
Abstract
BACKGROUND/AIMS:
Deoxynivalenol (DON) is a mycotoxin produced by Fusarium species which is commonly found in temperate regions worldwide as a natural contaminant of cereals. It is of great concern not only in terms of economic losses but also in terms of animal and public health. The digestive tract is the first and main target of this food contaminant and it represents a major site of immune tolerance. A finely tuned cross-talk between the innate and the adaptive immune systems ensures the homeostatic equilibrium between the mucosal immune system and commensal microorganisms. The aim of this study was to analyze the impact of DON on the intestinal immune response.
METHODOLOGY:
Non-transformed intestinal porcine epithelial cells IPEC-1 and porcine jejunal explants were used to investigate the effect of DON on the intestinal immune response and the modulation of naive T cells differentiation. Transcriptomic proteomic and flow cytometry analysis were performed.
RESULTS:
DON induced a pro-inflammatory response with a significant increase of expression of mRNA encoding for IL-8, IL-1α and IL-1β, TNF-α in all used models. Additionally, DON significantly induced the expression of genes involved in the differentiation of Th17 cells (STAT3, IL-17A, IL-6, IL-1β) at the expenses of the pathway of regulatory T cells (Treg) (FoxP3, RALDH1). DON also induced genes related to the pathogenic Th17 cells subset such as IL-23A, IL-22 and IL-21 and not genes related to the regulatory Th17 cells (rTh17) such as TGF-β and IL-10.
CONCLUSION:
DON triggered multiple immune modulatory effects which could be associated with an increased susceptibility to intestinal inflammatory diseases.
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