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Equine IL-17A (Yeast-derived Recombinant Protein) - 25 micrograms

RP0078E-025
$300.00
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Bulk quantities of proteins are available. Please contact us for bulk pricing.

IL-17A is a member of the IL-17 family, which is comprised of 6 members [IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F]. IL-17 family members are involved in numerous immune regulatory functions, including inducing and mediating proinflammatory responses and allergic responses. Il-17 induces the production of many other cytokines (IL-6, G-CSF, GM-CSF, IL-1beta, TGF-beta, and TNF-alpha), chemokines, including IL-8 (CXCL8), GRO-alpha (CXCL1) and MCP-1 (CCL2) and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages).

Alternate Names - IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A

IL-17A Homology Across Species
Equus caballus (horse) IL-17A – 100%
Equus przewalskii (Przewalski's horse) IL-17A – 99%
Equus asinus (ass) IL-17A – 98%
More… https://blast.ncbi.nlm.nih.gov

Equine IL-17A (Yeast-derived Recombinant Protein) - 25 micrograms
Catalog No.:
RP0078E-025
Quantity:
25 ug
Source:
The Equine IL-17A recombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
The Equine IL-17A recombinant protein has a predicted molecular weight of 14.9 kDa.
Protein Sequence:
GIVIPQNPEC PNTGDKNFPQ NVKINLNVLN RKTNSRRASD YHNRSTSPWN LHRNEDPERY PSVIWEAKCR HLGCVNAEGK VDFHMNSVPI QQEILVLRRE SQNCPHSFQL EKMLVAVGCT CVTPIVRHMG (130)
Country of Origin:
USA
Applications:
The IL-17A endotoxin-free recombinant protein can be used in cell culture, as a IL-17A ELISA Standard, and as a Western Blot Control.

22051225

Characterization of cytokines associated with Th17 cells in the eyes of horses with recurrent uveitis.

Regan DP, Aarnio MC, Davis WS, Carmichael KP, Vandenplas ML, Lauderdale JD, Moore PA.

Vet Ophthalmol. 2012 May;15(3):145-52.

Applications: Positive Control in Western Blot

Abstract
OBJECTIVE:
Equine recurrent uveitis (ERU) is a spontaneous disease that is the most common cause of blindness in horses, affecting up to 15% of the horse population. Th17 cells are a major cell population driving the pathogenesis in several mouse models of autoimmune inflammation, including experimental autoimmune uveitis. The purpose of this study is to investigate the role a Th17 cell-mediated response plays in the pathogenesis of ERU.
PROCEDURE:
Banked, Davidson's-fixed equine globes histopathologically diagnosed with ERU (n = 7) were compared immunohistochemically with healthy control globes (n = 7). Immunohistochemical staining was performed using a pan-Leptospira antibody and antibodies against IL-6, IL-17, and IL-23. Additionally, immunostaining was performed for T-cell (CD3) and B-cell (CD79α) markers. Specificity of immunoreactivity was confirmed by western blot analysis.
RESULTS:
Immunohistochemical staining was positive for IL-6, IL-17, and IL-23 within the cytoplasm of nonpigmented ciliary epithelial cells and mononuclear inflammatory cells infiltrating the iris, and ciliary body of ERU horses (n = 7) but negative in controls (n = 7). ERU-affected eyes were CD3 positive (n = 7) and CD79α negative (n = 7). Staining for Leptospira was negative in all ERU and control globes.
CONCLUSIONS:
Strong immunoreactivity for IL-6, IL-17, and IL-23, in conjunction with the fact that T lymphocytes are the predominating inflammatory cells present in ERU, suggests that IL-17-secreting helper T-cells play a role in the pathogenesis of ERU. These findings suggest that horses with ERU may serve as a naturally occurring animal model for autoimmune uveitis.


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