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Chicken IL-6 (Yeast-derived Recombinant Protein) - 100 micrograms

RP0299C-100
$900.00
In Stock
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Bulk quantities of Chicken IL-6 protein are available.
Please contact us for pricing.

Chicken IL-6 Specifications

Molecular Weight (calculated) - 22.0kDa

Amino Acid Sequence - VPLPAAADSS GEVGLEEEAG ARRALLDCEP LARVLRDRAV QLQDEMCKKF TVCENSMEML VRNNLNLPKV TEEDGCLLAG FDEEKCLTKL SSGLFAFQTY LEFIQETFDS EKQNVESLCY STKHLAATIR QMVINPDEVV IPDSAAQKSL LANLKSDKDW IEKITMHLIL RDFTSFMEKT VRAVRYLKKT RSFSA (195)

Gene ID - 395337

Homology Across Species
Gallus gallus (chicken) IL-6 - 100%
More - https://blast.ncbi.nlm.nih.gov/

Endotoxin - Naturally endotoxin-free

Applications

Cell Culture, ELISA Standard, Western Blot Control

IL-6 Background

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also produced from muscle, and is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.

Alternate Names - IL6, BSF2, HGF, HSF, IFNB2, IL-6, BSF-2, CDF, IFN-beta-2, interleukin 6

Catalog No.:
RP0299C-100
Quantity:
100 ug
Source:
The Chicken IL-6 recoombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
The Chicken IL-6 recombinant protein has a predicted molecular weight of 22.0 kDa.
Protein Sequence:
VPLPAAADSS GEVGLEEEAG ARRALLDCEP LARVLRDRAV QLQDEMCKKF TVCENSMEML VRNNLNLPKV TEEDGCLLAG FDEEKCLTKL SSGLFAFQTY LEFIQETFDS EKQNVESLCY STKHLAATIR QMVINPDEVV IPDSAAQKSL LANLKSDKDW IEKITMHLIL RDFTSFMEKT VRAVRYLKKT RSFSA (195)
Country of Origin:
USA
Applications:
The Chicken IL-6 endotoxin-free recombinant protein can be used in cell culture, as an IL-6 ELISA Standard, and as a Western Blot Control.

32183481

Chlamydia psittaci PmpD-N Exacerbated Chicken Macrophage Function by Triggering Th2 Polarization and the TLR2/MyD88/NF-κB Signaling Pathway

Chu J, Li X, Qu G, Wang Y, Li Q, Guo Y, Hou L, Liu J, Eko FO, He C.

Int J Mol Sci. 2020 Mar 15;21(6):2003. doi: 10.3390/ijms21062003.

Applications: Measurement of chicken IL-2, IL-6, IL-10, IL-12, and IFN-γ in cell culture supernatants by ELISA

Abstract

The polymorphic membrane protein D (PmpD) is a highly conserved outer membrane protein which plays an important role in pathogenesis during Chlamydia psittaci infection. In this study, we evaluated the ability of the N-terminus of PmpD (PmpD-N) to modulate the functions of chicken macrophages and the signaling pathway(s) involved in PmpD-N-induced Toll-like receptors (TLRs), as well as interleukin (IL)-6 and IL-10 cytokine secretions. Thus, HD11 macrophages were treated with exogenous and intracellular PmpD-N of C. psittaci. The chlamydial growth was evaluated by enumeration of chlamydial loads in the infected macrophages. The phagocytic function of macrophages following PmpD-N treatment was detected by fluorescein-labeled Escherichia coli (E. coli). The concentration of nitric oxide (NO) secreted by HD11 macrophages was measured by the amount of NO2- in the culture supernatant using the Griess method. The cytokine secretions were assessed using multiplex cytokine ELISA kits. Expression levels of TLRs, myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B (NF-κB) were analyzed by a Western blotting assay, as well as a luciferase assay, while NF-κB p65 nuclear translocation was assessed by confocal microscopy. The nuclear translocation of the transcription factor NF-κB was confirmed by evaluating its ability to combine with the corresponding promoter using the electrophoretic mobility shift assay (EMSA). After treatment with exogenous or endogenous PmpD-N, chlamydial loads and phagocytic functions were reduced significantly compared with those of the plasmid vector group, while NO secretions were reduced significantly compared with those of the lipopolysaccharide (LPS) treatment. Stimulation of HD11 cells with PmpD-N provoked the secretion of the Th2 cytokines, IL-6, and IL-10 and upregulated the expression of TLR2, TLR4, MyD88, and NF-κB. Furthermore, inhibition of TLR2, MyD88, and NF-κB in HD11 cells significantly decreased IL-6 and IL-10 cytokine levels, while NO production and phagocytosis increased significantly, strongly suggesting their involvement in PmpD-N-induced Th2 cytokine secretion and macrophage dysfunction. Our data indicate that C. psittaci PmpD-N inhibited macrophage functions by activating the Th2 immune response and the TLR2/MyD88/NF-κB signaling pathway.

 


31951466

Feed-borne Bacillus cereus Exacerbates Respiratory Distress in Chickens Infected With Chlamydia psittaci by Inducing Haemorrhagic Pneumonia.

Zuo Z, Li Q, Guo Y, Li X, Huang S, Hegemann JH, He C.

Avian Pathol. 2020 Jun;49(3):251-260. doi: 10.1080/03079457.2020.1716940. Epub 2020 Mar 12.

Applications: Measurement of chicken IL-6 nd IL-10 in lung lavage samples by ELISA

Abstract

Chlamydia psittaci is an important zoonotic pathogen and its oral route of infection plays an important role in the transmission and persistence. Bacillus cereus (B. cereus) strain, a common contaminant of animal feed and feedstuffs, can cause severe diarrhoea and malnutrition in poultry. In our previous study, a B. cereus strain (Dawu C), isolated from the haemorrhagic lungs of infected chickens, was shown to harbour two virulence genes (hblC and cytk) and was able to induce haemorrhagic lesions in the lungs, as well as gizzard erosion and ulceration (GEU) syndrome in broilers. In the present study, we tested the hypothesis that B. cereus-induced GEU would aggravate C. psittaci infection. Our results showed that SPF chickens exposed to B. cereus developed a severe GEU syndrome. More interestingly, prior infection with B. cereus facilitated C. psittaci infection, and aggravated GEU and respiratory distress, which were accompanied by high chlamydial loads in the lungs and severe lesions in respiratory organs. Moreover, levels of local inflammatory cytokines were elevated and T cell responses were impaired in the infected birds. In conclusion, GEU caused by B. cereus may facilitate chlamydial transmission from the ventriculus to the lungs.

 


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