Component | Use | Quantity | Catalog # |
Anti-Bovine IFNβ Polyclonal Antibody | Capture Antibody | 100 µg X 2 | PB0444B-100 |
Biotinylated Anti-Bovine IFNβ Polyclonal Antibody | Detection Antibody | 50 µg | PBB0450B-050 |
Bovine IFNβ Recombinant Protein | Standard | 5 µg | RP0298B-005 |
The Bovine IFNβ Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Bovine IFNβ ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Type I interferons comprise a vast and growing group of IFN proteins. Homologous molecules to type I IFNs are found in many species, including all mammals, and some have been identified in birds, reptiles, amphibians and fish species. The mammalian types are designated IFN-α, IFN-β, IFN-κ, IFN-δ, IFN-ε, IFN-τ, IFN-ω, and IFN-ζ (also known as limitin). They are mainly involved in innate immune response against viral infection.
Alternate Names - IFB; IFF; IFNB; IFN-beta
Increased Susceptibility of Cattle to Intranasal RVFV Infection.
Kroeker AL, Smid V, Embury-Hyatt C, Collignon B, Pinette M, Babiuk S, Pickering B.
Front Vet Sci. 2020 Apr 29;7:137. doi: 10.3389/fvets.2020.00137. eCollection 2020.
Applications: Measurement of bovine IFN alpha and IFN beta in nasal and oral swabs by ELISA.
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