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CartCanine IL-1 beta (IL-1F2) Do-It-Yourself ELISA, ≤20 Plates
Canine IL-1β ELISA Data
Canine IL-1β ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Canine IL-1β (IL-1F2) Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1015D-100 |
| Biotinylated Anti-Canine IL-1β (IL-1F2) Polyclonal Antibody | Detection Antibody | 50 µg | KPB1016D-050 |
| Canine IL-1β (IL-1F2) Recombinant Protein | Standard | 5 µg | RP0085D-005 |
Canine IL-1β ELISA Specifications
The Canine IL-1β Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Canine IL-1β ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Canine IL-1β Do-It-Yourself ELISA can also be used to measure Dingo IL-1β.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-1β Background
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Alternate Names - IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta
Interleukin-1β expression is increased in the duodenum of dogs with idiopathic inflammatory bowel disease.
Hawes M, Riddle A, Kirk J, Jergens A, Allenspach K.
Vet Rec. 2018 Nov 3;183(17):536. doi: 10.1136/vr.104495. Epub 2018 May 9.
Applications: Measurement of canine IL-1 beta in tissue homogenates supernatant in dogs with IBD by ELISA.
Cytokine and Growth Factor Concentrations in Canine Autologous Conditioned Serum.
Sawyere DM, Lanz OI, Dahlgren LA, Barry SL, Nichols AC, Werre SR.
Vet Surg. 2016 Jul;45(5):582-6. doi: 10.1111/vsu.12506.
Applications: Measurement of canine IL-1RA, IL-1 beta, and TNF alpha in serum and plasma by ELISA
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