Component | Usage | Quantity | Catalog # |
Anti-Equine CCL3 (MIP-1α) Polyclonal Antibody | Capture Antibody | 100 µg X 2 | PB0400E-100 |
Biotinylated Anti-Equine CCL3 (MIP-1α) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0401E-050 |
Equine CCL3 (MIP-1α) Recombinant Protein | Standard | 5 µg | RP0065E-005 |
The Equine CCL3 (MIP-1α) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Equine CCL3 (MIP-1α) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Chemokine ligand 3 (CCL3), also known as Macrophage Inflammatory Protein-1 alpha (MIP-1α) is a small cytokine belonging to the CC chemokine family. There are at least 27 distinct members of the C-C subgroup reported for mammals. They are characterized by two adjacent cysteines. CC chemokines induce the migration of monocytes and other cell types such as NK cells and dendritic cells. CCL3/MIP-1α is a chemoattractant for several different leukocytes, with varying degrees of potency (monocytes = T-cells>neutrophils>eosinophils).
Alternate Names - CCL3, G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3, C-C motif chemokine ligand 3
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