Component | Usage | Quantity | Catalog # |
Anti-Equine IL-1α (IL-1F1) Polyclonal Antibody | Capture Antibody | 100 µg X 2 | PB0471E-100 |
Biotinylated Anti-Equine IL-1α (IL-1F1) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0480E-050 |
Equine IL-1α (IL-1F1) Recombinant Protein | Standard | 5 µg | RP0358E-005 |
The Equine IL-1α (IL-1F1) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Equine IL-1α ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additionl tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Alternate Names - IL1A, IL-1A, IL1, IL1-ALPHA, IL1F1, interleukin 1 alpha, IL-1 alpha, IL-1α
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