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CartEquine IL-1 beta (IL-1F2) Do-It-Yourself ELISA, ≤20 Plates
Equine IL-1β ELISA Data
Equine IL-1β ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Equine IL-1β (IL-1F2) Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1013E-100 |
| Biotinylated Anti-Equine IL-1β (IL-1F2) Polyclonal Antibody | Detection Antibody | 50 µg | KPB1014E-050 |
| Equine IL-1β (IL-1F2) Recombinant Protein | Standard | 5 µg | RP0060E-005 |
Equine IL-1β ELISA Specifications
The Equine IL-1β Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Equine IL-1β ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-1β Background
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Alternate Names - IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta
Effects of Orally Administered Resveratrol on TNF, IL-1β, Leukocyte Phagocytic Activity and Oxidative Burst Function in Horses: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Study.
Martin LM, Johnson PJ, Amorim JR, DeClue AE.
Int J Mol Sci. 2020 Feb 20;21(4). pii: E1453. doi: 10.3390/ijms21041453.
Applications: Measurement of equine IL-1 beta in cell culture supernatant by ELISA
Abstract
Resveratrol, a phytophenol, is a commonly used equine nutraceutical supplement touted to exert anti-inflammatory effects. The effect of orally administered resveratrol on tumor necrosis factor (TNF), interleukin-1β (IL-1β), leukocyte phagocytic activity or oxidative burst function have not been reported in horses. The objective of this study was to determine the effects of a commercially available, orally administered resveratrol product on innate immune functions in healthy adult horses. Whole blood was collected from 12 horses prior to and following 3 weeks of treatment with either the manufacturer's recommended dose of resveratrol or placebo. Phagocytosis, oxidative burst and pathogen associated molecular pattern (PAMP) motif-stimulated leukocyte production of TNF and IL-1β were compared pre- and post-treatment between treatment groups. Phagocytosis and oxidative burst capacity were evaluated via flow cytometry. Tumor necrosis factor and IL-1β were measured using cytotoxicity and ELISA assays, respectively. There were no significant differences in phagocytosis, oxidative burst or stimulated TNF or IL-1β production between resveratrol and placebo treatment groups. Orally administered resveratrol at a routinely recommended dose for a duration of 3 weeks did not significantly affect phagocytic activity, oxidative burst function or PAMP-stimulated leukocyte cytokine production.
Feeding Grass Hay Before Concentrate Mitigates the Effect of Grain-Based Concentrates on Postprandial Plasma Interleukin-1β.
Suagee-Bedore JK, Linden DR, Bennett-Wimbush K, Splan RK.
J Equine Vet Sci. 2020 Mar;86:102899. doi: 10.1016/j.jevs.2019.102899. Epub 2019 Dec 24.
Applications: Measurement of equine IL-1 beta in plasma by ELISA
Increasing platelet concentrations in leukocyte-reduced platelet-rich plasma decrease collagen gene synthesis in tendons.
Boswell SG, Schnabel LV, Mohammed HO, Sundman EA, Minas T, Fortier LA.
Am J Sports Med. 2014 Jan;42(1):42-9. doi: 10.1177/0363546513507566. Epub 2013 Oct 17.
Applications: Measurement of equine IL-1 beta in platelet-rich plasma by ELISA
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