Component | Usage | Quantity | Catalog # |
Anti-Mouse IL-17A Polyclonal Antibody | Capture Antibody | 100 µg X 2 | PB0496M-100 |
Biotinylated Anti-Mouse IL-17A Polyclonal Antibody | Detection Antibody | 50 µg | PBB0497M-050 |
Rat IL-17A Recombinant Protein | Standard | 5 µg | RP0457R-005 |
The Rat IL-17A Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Rat IL-17A ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-17A is a member of the IL-17 family, which is comprised of 6 members [IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F]. IL-17 family members are involved in numerous immune regulatory functions, including inducing and mediating proinflammatory responses and allergic responses. IL-17 induces the production of many other cytokines (IL-6, G-CSF, GM-CSF, IL-1β, TGF-β, and TNF-α), chemokines, including IL-8 (CXCL8), GRO-α (CXCL1) and MCP-1 (CCL2) and prostaglandins from many cell types (fibroblasts, endothelial cells, epithelial cells, keratinocytes and macrophages).
Alternate Names - IL17A, CTLA8, IL-17, IL-17A, IL17, CTLA-8, interleukin 17A
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