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CartSwine Erythropoietin (EPO) Do-It-Yourself ELISA, ≤20 Plates
Swine EPO ELISA Data
Swine EPO ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Swine Erythropoietin Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1003S-100 |
| Biotinylated Anti-Swine Erythropoietin Polyclonal Antibody | Detection Antibody | 50 µg | KPB1004S-050 |
| Swine Erythropoietin Recombinant Protein | Standard | 5 µg | RP0938S-005 |
Swine EPO ELISA Specifications
The Swine Erythropoietin Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Swine EPO ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
EPO Background
Erythropoietin (EPO) is an essential hormone for red blood cell production. Without it, definitive erythropoiesis does not take place. Erythropoietin has its primary effect on red blood cell progenitors and precursors by promoting their survival through protecting these cells from apoptosis. It is the primary erythropoietic factor that cooperates with various other growth factors (e.g., IL-3, IL-6, glucocorticoids, and SCF) involved in the development of erythroid lineage from multipotent progenitors. Erythropoietin has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It can increase iron absorption by suppressing the hormone hepcidin. EPO is highly glycosylated, with half-life in blood around five hours. The half-life of EPO may vary between endogenous and various recombinant versions. Additional glycosylation or other alterations of EPO via recombinant technology have led to increased stability in blood. EPO binds to the erythropoietin receptor on the red cell progenitor surface and activates a JAK2 signaling cascade. Erythropoietin receptor expression is found in a number of tissues, such as bone marrow and peripheral/central nervous tissue. In the bloodstream, red cells themselves do not express erythropoietin receptor, so cannot respond to EPO. However, indirect dependence of red cell longevity in the blood on plasma erythropoietin levels has been reported, a process termed neocytolysis.
Alternate Names - EPO, EP, MVCD2, erythropoietin, Erythropoietin, ECYT5, DBAL
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Kingfisher Biotech, Inc.
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