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CartBovine IL-2 Do-It-Yourself ELISA, ≤10 Plates
Bovine IL-2 ELISA Data
Bovine IL-2 ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Bovine IL-2 Polyclonal Antibody | Capture Antibody | 100 µg | PB0121B-100 |
| Biotinylated Anti-Bovine IL-2 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1099B-050 |
| Bovine IL-2 Recombinant Protein | Standard | 5 µg | RP0026B-005 |
Bovine IL-2 ELISA Specifications
The Bovine IL-2 Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Bovine IL-2 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine IL-2 Do-It-Yourself ELISA can also be used to measure Bison, Water Buffalo, Wild Yak, and Zebu IL-2.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-2 Background
Interleukin-2 (IL-2) is a cytokine produced by T-helper cells in response to antigenic or mitogenic stimulation. It is required for T-cell proliferation and other activities crucial to the regulation of the immune response. IL-2 was discovered to be a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and a common gamma chain (γc). All members of this family use the common gamma chain as part of their signaling complex.
Alternate Names - IL2, IL-2, TCGF, lymphokine, interleukin 2
Lactation stage impacts the glycolytic function of bovine CD4+ T cells during ex vivo activation.
Eder JM, Gorden PJ, Lippolis JD, Reinhardt TA, Sacco RE.
Sci Rep. 2020 Mar 4;10(1):4045. doi: 10.1038/s41598-020-60691-2.
Applications: Measurement of bovine TNF alpha, IL-2, and IFN gamma in culture supernatants by ELISA
Abstract
Dairy cattle undergo dynamic physiological changes over the course of a full lactation into the dry period, which impacts their immunocompetence. During activation, T cells undergo a characteristic rewiring to increase the uptake of glucose and metabolically reprogram to favor aerobic glycolysis over oxidative phosphorylation. To date it remains to be completely elucidated how the altered energetic demands associated with lactation in dairy cows impacts T cell metabolic reprogramming. Thus, in our ex vivo studies we have examined the influence of stage of lactation (early lactation into the dry period) on cellular metabolism in activated bovine CD4+ T cells. Results showed higher rates of glycolytic function in activated CD4+ T cells from late lactation and dry cows compared to cells from early and mid-lactation cows. Similarly, protein and mRNA expression of cytokines were higher in CD4+ T cells from dry cows than CD4+ T cells from lactating cows. The data suggest CD4+ T cells from lactating cows have an altered metabolic responsiveness that could impact the immunocompetence of these animals, particularly those in early lactation, and increase their susceptibility to infection.
The chronic stages of bovine Fasciola hepatica are dominated by CD4 T-cell exhaustion
Sachdev D, Gough KC, Flynn RJ.
J Immunol. 2014 Jul 15;193(2):597-609
Applications: Bovine IL-2 was used to stimulate cells. Bovine IL-2 and IL-13 were measure in cell culture supernatants by ELISA.
Abstract
Fasciola hepatica infection of ruminants leads to non-resolving chronic infection, as patency develops, there is switching to a TGF-β and IL-10 led response. Here, we explore the responses of CD4 T-cells within the major draining lymph nodes. We found minimal expression of Foxp3 within CD4 cells but elevated levels within the γδ (WC1+) population. There is a strong T-cell-intrinsic exhaustion phenotype within the hepatic lymph node (HLN) characterized by a lack of antigen-specific proliferation and cytokine secretion. CD4 T-cells recovered from the HLN had high levels of PD-1 expression and low levels of IL-2 secretion. Exogenous IL-2 partially rescued this defect; when combined with neutralization of IL-10 and TGF-β, full restoration of proliferation, and cytokine production was achieved. Moreover, there is a clear uncoupling of the mechanisms that facilitate this regulation with parasite-specific proliferation and cytokine secretion being governed by independent means. These data would suggest that there is a CD4 T-cell-intrinsic regulation in place early in chronic infection, potentially leading to failure in resistance to reinfection.
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