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CartFeline CD40 Ligand Do-It-Yourself ELISA, ≤20 Plates
Feline CD40 Ligand ELISA Data
Feline CD40 Ligand ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Bovine CD40 Ligand Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1209B-100 |
| Biotinylated Anti-Bovine CD40 Ligand Polyclonal Antibody | Detection Antibody | 50 µg | KPB1210B-050 |
| Feline CD40 Ligand Recombinant Protein | Standard | 5 µg | RP0988F-005 |
Feline CD40 Ligand ELISA Specifications
The Feline CD40 Ligand Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a CD40 Ligand ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Feline CD40 Ligand Do-It-Yourself ELISA can also be used to measure Amur Tiger, Cheetah, and Leopard CD40 Ligand.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
CD40 Ligand Background
CD40 Ligand (CD40LG) is a transmembrane protein that is a member of the TNF superfamily. In addition to existing in a membrane bound form, CD40LG can also be released as a soluble molecule (sCD40LG), which has been shown to have biological functions.
CD40 interacts with CD40 ligand (aka CD40LG, TNFSF5 or CD154), which is predominantly expressed on activated CD4+ T-cells. CD40LG is transiently expressed on activated T-cells and plays a crucial role in B-cell function. Activated T-cells expressing CD40LG can also interact with CD40 on endothelial cells to induce production of inflammatory cytokines. The transient expression of CD40LG allows T-cells to stimulate selected CD40-bearing cells to participate in the immune response. Increased levels of sCD40LG have been measured in patients with SLE, RA, inflammatory bowel diseases, systemic sclerosis, and Sjogren's syndrome. In culture, canine sCD40LG was shown to promote the maturation and activation of canine monocyte derived Dendritic cells.
Alternate Names - CD40LG, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand
Ordering Information & Terms and Conditions
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Kingfisher Biotech, Inc.
1000 Westgate Drive
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Saint Paul, MN 55114
USA
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