Component | Usage | Quantity | Catalog # |
Anti-Equine IL-12 (IL-23) p40 Polyclonal Antibody | Capture Antibody | 100 µg | KP1225E-100 |
Biotinylated Anti-Equine IL-12 (IL-23) p40 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1226E-050 |
Equine IL-12 (IL-23) p40 Recombinant Protein | Standard | 5 µg | RP0924E-005 |
The Equine IL-12 (IL-23) p40 Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Equine IL-12 (IL-23) p40 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-12 is a member of the IL-12 family, which includes IL-12, IL-23, IL-27, and IL-35. Like other IL-12 family members, IL-12 is a heterodimeric cytokine. IL-12 is encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer, and a homodimer of p40 are formed following protein synthesis. IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ.
Alternate Names - IL12B, CLMF, CLMF2, IL-12B, IMD28, NKSF, NKSF2, IMD29, Interleukin 12 subunit beta, interleukin 12B
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