Component | Use | Quantity | Catalog # |
Anti-Zebrafish IFNγ 1-1 Polyclonal Antibody | Capture Antibody | 100 µg | KP1233Z-100 |
Biotinylated Anti-Zebrafish IFNγ 1-1 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1234Z-050 |
Zebrafish IFNγ 1-1 Recombinant Protein | Standard | 5 µg | RP1058Z-005 |
The Zebrafish IFN-γ 1-1 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Zebrafish IFNγ 1-1 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Interferon-gamma (IFN-γ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. This interferon was originally called macrophage-activating factor, a term now used to describe a larger family of proteins to which IFN-γ belongs. IFN-γ, or type II interferon, is a cytokine that is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, but, most important, derives from its immunostimulatory and immunomodulatory effects. IFN-γ is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.
Alternate Names - IFNG, IFG, IFI, interferon, gamma, interferon gamma
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