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CartZebrafish VEGF-A Do-It-Yourself ELISA, ≤20 Plates
Zebrafish VEGF-A ELISA Data
Zebrafish VEGF-A ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Zebrafish VEGF-A Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1270Z-100 |
| Biotinylated Anti-Zebrafish VEGF-A Polyclonal Antibody | Detection Antibody | 50 µg | KPB1271Z-050 |
| Zebrafish VEGF-A Recombinant Protein | Standard | 5 µg | RP1040Z-005 |
Zebrafish VEGF-A ELISA Specifications
The Zebrafish VEGF-A Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Zebrafish VEGF-A ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
VEGF-A Background
Vascular endothelial growth factor (VEGF) proteins stimulate vasculogenesis and angiogenesis. They are part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. The normal function of VEGF proteins is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. The VEGF family has six members, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placental Growth Factor (PGF). Activity of VEGF-A, as its name implies, has been studied mostly on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor (VPF).
Alternate Names - VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A
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Kingfisher Biotech, Inc.
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Kingfisher Biotech brand products are warranted by Kingfisher Biotech, Inc. to meet stated product specifications and to conform to label descriptions when used, handled and stored according to instructions. Unless otherwise stated, this warranty is limited to one year from date of sale. Kingfisher Biotech’s sole liability for the product is limited to replacement of the product or refund of the purchase price. Kingfisher Biotech brand products are supplied for research applications. They are not intended for medicinal, diagnostic or therapeutic use. The products may not be resold, modified for resale or used to manufacture commercial products without prior written approval from Kingfisher Biotech.
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Returns
Please call customer service before returning any products for refund, credit or replacement. NO returns will be accepted without prior written authorization. Returns are subject to a restocking fee of 20%.