Component | Usage | Quantity | Catalog # |
Anti-Canine Fas Ligand (FasL) Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1286D-100 |
Biotinylated Anti-Canine Fas Ligand (FasL) Polyclonal Antibody | Detection Antibody | 50 µg | KPB1287D-050 |
Canine Fas Ligand (FasL) Recombinant Protein | Standard | 5 µg | RP1088D-005 |
The Canine Fas Ligand (FasL) Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Canine Fas Ligand (FasL) ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Canine Fas Ligand (FasL) Do-It-Yourself ELISA can also be used to measure Dingo, and Red Fox Fas Ligand.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Fas ligand (FasL), also known as CD95L and TNFSF6, is a homotrimeric type II transmembrane protein belonging to the tumor necrosis factor (TNF) superfamily. FasL plays diverse roles in the immune system, including induction of apoptotic cell death in target cells. FasL-Fas-mediated apoptosis plays fundamental roles in several immune functions. The pathway is involved in functions including regulation of T cell homeostasis and maintenance of immune privilege, apart from its well-established role in T cell-mediated cytotoxicity. Cytotoxicity via the Fas pathway is critical for prevention of pathogenic infections as well as control of tumor cell growth
Alternate Names - FASLG, ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, TNFSF6, TNLG1A, Fas ligand
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