Component | Usage | Quantity | Catalog # |
Anti-IGF-1 Polyclonal Antibody | Capture Antibody | 100 µg X 2 | PB1331-100 |
Biotinylated Anti-IGF-1 Polyclonal Antibody | Detection Antibody | 50 µg | PBB1332-050 |
IGF-1 Recombinant Protein | Standard | 5 µg | RP1330-005 |
The IGF-1 Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a IGF-1 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IGF-1, also called somatomedin C, is is a primary mediator of the effects of growth hormone (GH). Growth hormone stimulates the liver to produce IGF-1. IGF-1 then stimulates systemic body growth, and has growth-promoting effects on almost every cell in the body, especially skeletal muscle, cartilage, bone, liver, kidney, nerves, skin, hematopoietic cell, and lungs. In addition to the insulin-like effects, IGF-1 can also regulate cell growth and development, especially in nerve cells, as well as cellular DNA synthesis.
This ELISA will measure IGF-1 of the following species since the protein sequence is 100% identical.
Ailuropoda melanoleuca (giant panda) IGF-1 – 100%
Bison bison bison (bison) IGF-1 – 100%
Bubalus bubalis (water buffalo) IGF-1 – 100%
Bos mutus (wild yak) IGF-1 – 100%
Bos indicus (zebu) IGF-1 – 100%
Camelus bactrianus (Bactrian camel) IGF-1 – 100%
Camelus ferus (Wild Bactrian camel) IGF-1 – 100%
Canis lupus familiaris (dog) IGF-1 – 100%
Capra hircus (goat) IGF-1 – 100%
Carlito syrichta (Philippine tarsier) IGF-1 – 100%
Cavia porcellus (domestic guinea pig) IGF-1 – 100%
Ceratotherium simum simum (southern white rhinoceros) IGF-1 – 100%
Cercocebus atys (sooty mangabey) IGF-1 – 100%
Chinchilla lanigera (long-tailed chinchilla) IGF-1 – 100%
Chlorocebus sabaeus (green monkey) IGF-1 – 100%
Dipodomys ordii (Ord's kangaroo rat) IGF-1 – 100%
Equus asinus (ass) IGF-1 – 100%
Equus caballus (horse) IGF-1 – 100%
Equus przewalskii (Przewalski's horse) IGF-1 – 100%
Felis catus (domestic cat) IGF-1 – 100%
Gorilla gorilla gorilla (western lowland gorilla) IGF-1 – 100%
Homo sapiens (human) IGF-1 – 100%
Ictidomys tridecemlineatus (thirteen-lined ground squirrel) IGF-1 – 100%
Leptonychotes weddellii (Weddell seal) IGF-1 – 100%
Lipotes vexillifer (Yangtze River dolphin) IGF-1 – 100%
Macaca fascicularis (Cynomolgus monkey) IGF-1 – 100%
Macaca mulatta (Rhesus monkey) IGF-1 – 100%
Macaca nemestrina (pig-tailed macaque) IGF-1 – 100%
Mandrillus leucophaeus (drill) IGF-1 – 100%
Manis javanica (Malayan pangolin) IGF-1 – 100%
Marmota marmota marmota (Alpine marmot) – 100%
Mustela putorius furo (domestic ferret) – 100%
Microcebus murinus (gray mouse lemur) – 100%
Nannospalax galili (Upper Galilee mountains blind mole rat) – 100%
Nomascus leucogenys (northern white-cheeked gibbon) – 100%
Odobenus rosmarus divergens (Pacific walrus) – 100%
Orcinus orca (killer whale) – 100%
Otolemur garnettii (small-eared galago) – 100%
Pan paniscus (pygmy chimpanzee) – 100%
Pan troglodytes (chimpanzee) – 100%
Panthera pardus (leopard) – 100%
Pantholops hodgsonii (chiru) – 100%
Papio anubis (olive baboon) – 100%
Physeter catodon (sperm whale) – 100%
Pongo abelii (Sumatran orangutan) – 100%
Pteropus vampyrus (large flying fox) – 100%
Rhinopithecus bieti (black snub-nosed monkey) – 100%
Rhinopithecus roxellana (golden snub-nosed monkey) – 100%
Rhinolophus sinicus (Chinese rufous horseshoe bat) – 100%
Sus scrofa (pig) – 100%
Tursiops truncatus (bottlenose dolphin) – 100%
Vicugna pacos (alpaca) – 100%
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