Component | Usage | Quantity | Catalog # |
Anti-Ovine IL-22 Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1418V-100 |
Biotinylated Anti-Ovine IL-22 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1419V-050 |
Ovine IL-22 Recombinant Recombinant Protein | Standard | 5 µg | RP1195V-005 |
The Ovine IL-22 Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Ovine IL-22 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-22 is a member of the IL-10 family, which includes IL-19, IL-20, IL-24, and IL-26. This family represents a class of potent mediators of cellular inflammatory responses. IL-22 shares use of IL-10R2 in cell signaling with other members of this family. IL-22 is produced by activated DC and T cells and initiates innate immune responses against bacterial pathogens, especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 along with IL-17 is rapidly produced by a subset of splenic cells and can also be produced by Th17 cells. IL-22 likely plays a role in the coordinated response of both adaptive and innate immune systems.
Alternate Names - IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18, interleukin 22
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