Component | Usage | Quantity | Catalog # |
Anti-Swine IL-3 Polyclonal Antibody | Capture Antibody | 100 µg | KP1421S-100 |
Biotinylated Anti-Swine IL-3 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1422S-050 |
Swine IL-3 Recombinant Protein | Standard | 5 µg | RP1298S-005 |
The Swine IL-3 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Swine IL-3 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-3 (Interleukin-3) stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. IL-3 also stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Alternate Names - IL3, interleukin 3, IL-3, MCGF, MULTI-CSF
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