Component | Usage | Quantity | Catalog # |
Anti-Mouse VEGF-A Polyclonal Antibody | Capture Antibody | 100 µg | KP0742M-100 |
Biotinylated Anti-Mouse VEGF-A Polyclonal Antibody | Detection Antibody | 50 µg | KPB0743M-050 |
Human & Monkey VEGF-A Recombinant Protein | Standard | 5 µg | RP1355HY-005 |
The Human & Monkey VEGF-A Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Human & Monkey VEGF-A ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Human/Monkey VEGF-A Do-It-Yourself ELISA can also be used to measure Sooty Mangabey, Green Monkey, Peter’s Angolan Colobus, Western Lowland Gorilla, Rhesus Monkey, Pig-tailed Macaque, Drill, Northern White-cheeked Gibbon, Pygmy Chimpanzee, Chimpanzee, Olive Baboon, and Golden Snub-nosed Monkey VEGF-A.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Vascular endothelial growth factor (VEGF) proteins stimulate vasculogenesis and angiogenesis. They are part of the system that restores the oxygen supply to tissues when blood circulation is inadequate. The normal function of VEGF proteins is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. The VEGF family has six members, including VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and Placental Growth Factor (PGF). Activity of VEGF-A, as its name implies, has been studied mostly on cells of the vascular endothelium, although it does have effects on a number of other cell types (e.g., stimulation monocyte/macrophage migration, neurons, cancer cells, kidney epithelial cells). In vitro, VEGF-A has been shown to stimulate endothelial cell mitogenesis and cell migration. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor (VPF).
Alternate Names - VEGFA, MVCD1, VEGF, VPF, vascular endothelial growth factor A
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