Component | Usage | Quantity | Catalog # |
Anti-Human EGF Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1471H-100 |
Biotinylated Anti-Human EGF Polyclonal Antibody | Detection Antibody | 50 µg | KPB1472H-050 |
Human EGF Recombinant Protein | Standard | 5 µg | RP1424H-005 |
The Human EGF-A Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Human EGF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Human EGF-A Do-It-Yourself ELISA can also be used to measure Chimpanzee, Pygmy Chimpanzee, and Western Lowlands Gorilla EGF.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
EGF (Epidermal Growth Factor) is best known for having the ability to stimulate cell growth and proliferation. There are many EGF responses and they are regulated by seven ligands: EGF, transforming growth factor-alpha (TGFA), heparin-binding EGF-like growth factor (HBEGF), betacellulin (BTC), amphiregulin (AREG), epiregulin (EREG), and epigen (EPGN) and four ErbB (HER) family receptor tyrosine kinases: EGFR/ErbB1, ErbB2, ErbB3 and ErbB4. EGF family members are important for development and promoting mitogenesis and differentiation of mesenchymal and epithelial cells. EGF acts as a mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. EGF plays a significant role in epithelial development, angiogenesis, regulation of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture. EGF dysregulation is also associated with the growth and progression of many cancers.
Alternate Names - EGF, HOMG4, URG, epidermal growth factor, Epidermal growth factor
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