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Caprine LIF Do-It-Yourself ELISA, ≤10 Plates

DIY1490G-003
$770.00
In Stock
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Caprine LIF ELISA Data

Caprine LIF Standard Curve

Caprine LIF ELISA Kit Components

Component Usage Quantity Catalog #
Anti-Human LIF Polyclonal Antibody Capture Antibody 100 µg KP1486H-100
Biotinylated Anti-Human LIF Polyclonal Antibody Detection Antibody 50 µg KPB1487H-050
Caprine LIF Recombinant Protein Standard 5 µg RP1075G-005

 

Caprine LIF ELISA Specifications

The Caprine LIF-A Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Caprine LIF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately. 

For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.

LIF Background

Leukemia inhibitory factor, or LIF, is a member of the IL-6 family. LIF affects cell growth by inhibiting differentiation. When LIF levels drop, the cells differentiate. Leukemia inhibitory factor derives its name from its ability to induce the terminal differentiation of myeloid leukemic cells, thus preventing their continued growth. Other properties attributed to LIF include: the growth promotion and cell differentiation of different types of target cells, influence on bone metabolism, cachexia, neural development, embryogenesis and inflammation. p53 regulated LIF has been shown to facilitate implantation in the mouse model and possibly in humans. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. LIF is typically added to stem cell culture medium to reduce spontaneous differentiation.

Alternate Names - LIF, CDF, DIA, HILDA, MLPLI, leukemia inhibitory factor, interleukin 6 family cytokine, LIF interleukin 6 family cytokine

Catalog No.:
DIY1490G-003
Quantity:
1 Pack
Alias:
Leukemia Inhibitory Factor
Country of Origin:
USA
Applications:
Measurement of Caprine LIF in an ELISA.
None at this time

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USA

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