Component | Usage | Quantity | Catalog # |
Anti-Bovine GM-CSF Polyclonal Antibody | Capture Antibody | 100 µg | KP1507B-100 |
Biotinylated Anti-Bovine GM-CSF Polyclonal Antibody | Detection Antibody | 50 µg | KPB1508B-050 |
Bovine GM-CSF Recombinant Protein | Standard | 5 µg | RP0871B-005 |
The Bovine GM-CSF Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Bovine GM-CSF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. Granulocyte macrophage–colony stimulating factor (GM-CSF) is secreted in response to inflammatory stimuli such as LPS, IL-1, and TNF-α by a variety of different cells, including endothelium, fibroblasts, muscle cells, and macrophages, and by activated T cells. GM-CSF is glycosylated in its mature form.
Alternate Names - CSF2, GMCSF, colony stimulating factor 2, CSF
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