Component | Usage | Quantity | Catalog # |
Anti-Bovine/Caprine/Ovine IL-5 Polyclonal Antibody | Capture Antibody | 100 µg | KP1617B-100 |
Biotinylated Anti-Bovine/Caprine/Ovine IL-5 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1618B-050 |
Bovine/Caprine/Ovine IL-5 Recombinant Protein | Standard | 5 µg | RP0139B-005 |
 
The Bovine/Caprine/Ovine IL-5 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine/Caprine/Ovine IL-5 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine/Caprine/Ovine IL-5 Do-It-Yourself ELISA can also be used to measure Bison and Zebu IL-5.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-3, IL-5, and GM-CSF are important in regulating eosinophil development. Of these three cytokines, IL-5 is the most specific to the eosinophil lineage and is responsible for selective terminal differentiation of eosinophils. The IL-5 receptor is composed of an alpha and a beta-common chain. The alpha subunit is specific for the IL-5 molecule, whereas the beta-common subunit also recognized by IL-3 and GM-CSF. IL-5 is produced by both hematopoietic and non-hematopoietic cells including T cells, granulocytes, and natural helper cells. It is associated with the cause of several allergic diseases including allergic rhinitis and asthma.
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