Component | Usage | Quantity | Catalog # |
Anti-Bovine/Caprine/Ovine IL-5 Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1617B-100 |
Biotinylated Anti-Bovine/Caprine/Ovine IL-5 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1618B-050 |
Bovine/Caprine/Ovine IL-5 Recombinant Protein | Standard | 5 µg | RP0139B-005 |
The Bovine/Caprine/Ovine IL-5 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine/Caprine/Ovine IL-5 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine/Caprine/Ovine IL-5 Do-It-Yourself ELISA can also be used to measure Bison and Zebu IL-5.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-3, IL-5, and GM-CSF are important in regulating eosinophil development. Of these three cytokines, IL-5 is the most specific to the eosinophil lineage and is responsible for selective terminal differentiation of eosinophils. The IL-5 receptor is composed of an alpha and a beta-common chain. The alpha subunit is specific for the IL-5 molecule, whereas the beta-common subunit also recognized by IL-3 and GM-CSF. IL-5 is produced by both hematopoietic and non-hematopoietic cells including T cells, granulocytes, and natural helper cells. It is associated with the cause of several allergic diseases including allergic rhinitis and asthma.
Please note that Cookies and JavaScript are required for you to view this website.
Check if you have Cookies and JavaScript enabled in your browser