Component | Usage | Quantity | Catalog # |
Anti-Ferret IL-8 Polyclonal Antibody | Capture Antibody | 100 µg | KP1954FT-100 |
Biotinylated Anti-Ferret IL-8 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1955FT-050 |
Ferret IL-8 Recombinant Protein | Standard | 5 µg | RP1741FT-005 |
The Ferret IL-8 Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Ferret IL-8 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Interleukin-8 (IL-8), also known as CXCL8, is a CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. There have been 17 different CXC chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2.
Alternate Names - CXCL8, chemokine (C-X-C motif) ligand 8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, IL8, C-X-C motif chemokine ligand 8, Interleukin-8
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