Component | Usage | Quantity | Catalog # |
Anti-Swine TWEAK Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP1966S-100 |
Biotinylated Anti-Swine TWEAK Polyclonal Antibody | Detection Antibody | 50 µg | KPB1967S-050 |
Swine TWEAK Recombinant Protein | Standard | 5 µg | RP0489S-005 |
The Swine TWEAK Do-It-Yourself ELISA contains capture antibody, recombinant protein standard, and detection antibody for development of a Swine TWEAK ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Tumor necrosis factor ligand superfamily member 12 (TNFSF12) also known as TNF-related weak inducer of apoptosis (TWEAK) is a member of the tumor necrosis factor ligand (TNF) ligand family. Nineteen cytokines have been identified as part of the TNF family on the basis of sequence, functional, and structural similarities. Family members include TNF beta (TNFSF1), TNF alpha (TNFSF2), Lymphotoxin beta (TNFSF3), OX40 Ligand (TNFSF4), CD40 Ligand (TNFSF5), Fas Ligand (TNFSF6), CD27 Ligand (TNFSF7), CD30 Ligand (TNFSF8), 4-1BB Ligand (TNFSF9), TRAIL (TNFSF10), TRANCE/RANKL (TNFSF11), TWEAK (TNFSF12), APRIL(TNFSF13), BAFF (TNFSF13B), LIGHT (TNFSF14), TL1A/VEGI (TNFSF15), and GITR Ligand (TNFSF18). TWEAK has overlapping signaling functions with TNF, but displays a much wider tissue distribution. TWEAK can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. TWEAK is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis.
Alternate Names - TNFSF12, APO3L, DR3LG, TWEAK, TNLG4A, tumor necrosis factor superfamily member 12, TNF superfamily member 12
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