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CartSwine IL-1 beta (IL-1F2) Do-It-Yourself ELISA, ≤10 Plates
Swine IL-1β ELISA Data
Swine IL-1β ELISA Kit Components
| Component | Usage | Quantity | Catalog # |
| Anti-Swine IL-1β (IL-1F2) Polyclonal Antibody | Capture Antibody | 100 µg | KP2021S-100 |
| Biotinylated Anti-Swine IL-1β (IL-1F2) Polyclonal Antibody | Detection Antibody | 50 µg | KPB2022S-050 |
| Bovine IL-1β (IL-1F2) Recombinant Protein | Standard | 5 µg | RP0297S-005 |
Swine IL-1β ELISA Specifications
The Swine IL-1β Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Swine IL-1β ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
IL-1β Background
The IL-1 family of cytokines encompasses eleven proteins that each share a similar β-barrel structure and bind to Ig-like receptors. Several of the well characterized members of the IL-1-like cytokines play key roles in the development and regulation of inflammation. IL-1α (IL-1F1), IL-1β (IL-1F2), and IL-18 (IL-1F4) are well-known inflammatory cytokines active in the initiation of the inflammatory reaction and in driving Th1 and Th17 inflammatory responses. In contrast, IL-1 receptor antagonist (IL-1ra; IL-1F3) and IL-36 receptor antagonist (IL-36ra; IL-1F5) reduce inflammation by blocking the binding of the agonist receptor ligands. IL-33 (IL-1F11) is thought to function as an 'alarmin' released following cell necrosis to alerting the immune system to tissue damage or stress. The biological properties of IL-37 (IL-1F7) are mainly those of down-regulating inflammation.
Alternate Names - IL1B, IL-1, IL1-BETA, IL1F2, interleukin 1 beta, IL1beta
Aerosol vaccination with Bacille CalmetteGuerin induces a trained innate immune phenotype in calves.
Guerra-Maupome M, Vang DX, McGill JL.
PLoS One. 2019 Feb 22;14(2):e0212751. doi: 10.1371/journal.pone.0212751. eCollection 2019.
Applications: Measurement of bovine TNF alpha, IL-1 beta, and IL-6 in culture supernatants by ELISA
Abstract
Mycobacterium bovis Bacillus Calmette-Guérin (BCG) is a live attenuated vaccine for use against tuberculosis (TB); however, it is known to reduce childhood mortality from infections other than TB. The unspecific protection induced by BCG vaccination has been associated with the induction of memory-like traits of the innate immune system identified as 'trained' immunity. In humans and mouse models, in vitro and in vivo BCG training leads to enhanced production of monocyte-derived proinflammatory cytokines in response to secondary unrelated bacterial and fungal pathogens. While BCG has been studied extensively for its ability to induce innate training in humans and mouse models, BCG's nonspecific protective effects have not been defined in agricultural species. Here, we show that in vitro BCG training induces a functional change in bovine monocytes, characterized by increased transcription of proinflammatory cytokines upon restimulation with the toll-like receptor agonists. Importantly, in vivo, aerosol BCG vaccination in young calves also induced a 'trained' phenotype in circulating peripheral blood mononuclear cells (PBMCs), that lead to a significantly enhanced TLR-induced proinflammatory cytokine response and changes in cellular metabolism compared to PBMCs from unvaccinated control calves. Similar to the long-term training effects of BCG reported in humans, our results suggest that in young calves, the effects of BCG induced innate training can last for at least 3 months in circulating immune populations. Interestingly, however, aerosol BCG vaccination did not 'train' the innate immune response at the mucosal level, as alveolar macrophages from aerosol BCG vaccinated calves did not mount an enhanced inflammatory response to secondary stimulation, compared to cells isolated from control calves. Together, our results suggest that, like mice and humans, the innate immune system of calves can be 'trained'; and that BCG vaccination could be used as an immunomodulatory strategy to reduce disease burden in juvenile food animals before the adaptive immune system has fully matured.
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