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CartHuman TNF alpha (TNFSF2) Do-It-Yourself ELISA, ≤10 Plates
Human TNFα ELISA Data
Human TNFα ELISA Kit Components
| Component | Use | Quantity | Catalog # |
| Anti-Canine TNFα (TNFSF2) Polyclonal Antibody | Capture Antibody | 100 µg | PB0389D-100 |
| Biotinylated Anti-Canine TNFα (TNFSF2) Polyclonal Antibody | Detection Antibody | 50 µg | PBB0397D-050 |
| Human TNFα (TNFSF2) Recombinant Protein | Standard | 5 µg | RP0440H-005 |
Specifications
The Human TNFα (TNFSF2) Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Human TNFα ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Human TNFα Do-It-Yourself ELISA can also be used to measure Chimpanzee TNFα.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Background
TNFα (TNFSF2) is a member of the TNF Superfamily. It is produced chiefly by activated macrophages, but it is produced also by a broad variety of cell types including lymphoid cells, mast cells, endothelial cells, cardiac myocytes, adipose tissue, fibroblasts, and neuronal tissue. The primary role of TNFα is in the regulation of immune cells. TNFα, being an endogenous pyrogen, is able to induce fever, to induce apoptotic cell death, to induce sepsis (through IL-1 & IL-6 production), to induce cachexia, induce inflammation, and to inhibit tumorigenesis and viral replication.
Alternate Names - TNF, DIF, TNF-alpha, TNFA, TNFSF2, Tumour necrosis factor, TNF-α, tumor necrosis factor, TNLG1F, Tumor necrosis factor alpha
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Kingfisher Biotech, Inc.
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Kingfisher Biotech brand products are warranted by Kingfisher Biotech, Inc. to meet stated product specifications and to conform to label descriptions when used, handled and stored according to instructions. Unless otherwise stated, this warranty is limited to one year from date of sale. Kingfisher Biotech’s sole liability for the product is limited to replacement of the product or refund of the purchase price. Kingfisher Biotech brand products are supplied for research applications. They are not intended for medicinal, diagnostic or therapeutic use. The products may not be resold, modified for resale or used to manufacture commercial products without prior written approval from Kingfisher Biotech.
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Returns
Please call customer service before returning any products for refund, credit or replacement. NO returns will be accepted without prior written authorization. Returns are subject to a restocking fee of 20%.