Component | Usage | Quantity | Catalog # |
Anti-Bovine M-CSF Polyclonal Antibody | Capture Antibody | 100 µg X 2 | KP2197B-100 |
Biotinylated Anti-Bovine M-CSF Polyclonal Antibody | Detection Antibody | 50 µg | KPB2198B-050 |
Caprine/Ovine M-CSF Recombinant Protein | Standard | 5 µg | RP1640G-005 |
The Caprine/Ovine M-CSF Do-It-Yourself ELISA contains capture antibody, standard, and detection antibody for development of a Caprine/Ovine M-CSF ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
Macrophage colony-stimulating factor (M-CSF), also known as CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF enhances expression of differentiation antigens and stimulates chemotactic, phagocytic and the killing activities of monocytes. M-CSF also stimulates production of several cytokines, including GM- CSF, G-CSF and IL-6 by priming monocytes. It also stimulates production and secretion of IL-8 and reactive nitrogen intermediates. In addition to the stimulation of hematopoiesis, M-CSF also stimulates differentiation and proliferation of osteoclast progenitor cells and cytotrophoblasts.
Alternate Names - CSF1, CSF-1, MCSF, colony stimulating factor 1
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