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CartChicken IFN gamma Polyclonal Antibody - Biotinylated
Reactivity - ELISA
Bovine IFN-γ - None
Canine IFN-γ - None
Chicken IFN-γ - Strong
Cynomolgus Monkey IFN-γ - None
Dolphin IFN-γ - None
Equine IFN-γ - None
Feline IFN-γ - None
Human IFN-γ - None
Mouse IFN-γ - None
Ovine IFN-γ - None
Rabbit IFN-γ - None
Swine IFN-γ - None
Zebrafish IFN-γ1-2 - None
Chicken IFN-γ ELISA Data
Chlamydia psittaci PmpD-N Exacerbated Chicken Macrophage Function by Triggering Th2 Polarization and the TLR2/MyD88/NF-κB Signaling Pathway
Chu J, Li X, Qu G, Wang Y, Li Q, Guo Y, Hou L, Liu J, Eko FO, He C.
Int J Mol Sci. 2020 Mar 15;21(6):2003. doi: 10.3390/ijms21062003.
Applications: Measurement of chicken IL-2, IL-6, IL-10, IL-12, and IFN-γ in cell culture supernatants by ELISA
Abstract
The polymorphic membrane protein D (PmpD) is a highly conserved outer membrane protein which plays an important role in pathogenesis during Chlamydia psittaci infection. In this study, we evaluated the ability of the N-terminus of PmpD (PmpD-N) to modulate the functions of chicken macrophages and the signaling pathway(s) involved in PmpD-N-induced Toll-like receptors (TLRs), as well as interleukin (IL)-6 and IL-10 cytokine secretions. Thus, HD11 macrophages were treated with exogenous and intracellular PmpD-N of C. psittaci. The chlamydial growth was evaluated by enumeration of chlamydial loads in the infected macrophages. The phagocytic function of macrophages following PmpD-N treatment was detected by fluorescein-labeled Escherichia coli (E. coli). The concentration of nitric oxide (NO) secreted by HD11 macrophages was measured by the amount of NO2- in the culture supernatant using the Griess method. The cytokine secretions were assessed using multiplex cytokine ELISA kits. Expression levels of TLRs, myeloid differentiation factor 88 (MyD88), and nuclear factor kappa B (NF-κB) were analyzed by a Western blotting assay, as well as a luciferase assay, while NF-κB p65 nuclear translocation was assessed by confocal microscopy. The nuclear translocation of the transcription factor NF-κB was confirmed by evaluating its ability to combine with the corresponding promoter using the electrophoretic mobility shift assay (EMSA). After treatment with exogenous or endogenous PmpD-N, chlamydial loads and phagocytic functions were reduced significantly compared with those of the plasmid vector group, while NO secretions were reduced significantly compared with those of the lipopolysaccharide (LPS) treatment. Stimulation of HD11 cells with PmpD-N provoked the secretion of the Th2 cytokines, IL-6, and IL-10 and upregulated the expression of TLR2, TLR4, MyD88, and NF-κB. Furthermore, inhibition of TLR2, MyD88, and NF-κB in HD11 cells significantly decreased IL-6 and IL-10 cytokine levels, while NO production and phagocytosis increased significantly, strongly suggesting their involvement in PmpD-N-induced Th2 cytokine secretion and macrophage dysfunction. Our data indicate that C. psittaci PmpD-N inhibited macrophage functions by activating the Th2 immune response and the TLR2/MyD88/NF-κB signaling pathway.
Application of Ecklonia cava Kjellman by-product as a feed additive: enhancing weight gain, immunity and protection from Salmonella infection in chickens.
Park, Soyeon, Kim, Chung Yoh, Park, Bokyoung, Kim, Kiju, Park, Keun-Tae, Han, Jong Kwon, & Hahn, Tae-Wook.
대한수의학회지, vol. 56, no. 4, 대한수의학회, Dec. 2016, pp. 255–260, doi:10.14405/KJVR.2016.56.4.255.
Applications: Measurement of chicken IFN gamma in cell culture supernatants by ELISA
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