Bovine IFN alpha A Recombinant Protein

Catalog Number:: RP0008B
Availability:: In stock
Application:: Cell Culture, ELISA Standard, ELISpot Control, Western Blot Control
100% Homology:: Bos taurus (cattle)

Bovine IFN-αA (catalog RP0008B) is a yeast-derived cytokine supplied lyophilized without carrier protein in 10% trehalose; it has no affinity tags and is naturally endotoxin-free, and should be reconstituted in sterile PBS that contains at least 0.1% carrier protein. The protein is ~19.0 kDa, 166 amino acids long (full sequence provided), and >98% pure by SDS-PAGE. Store at -20°C (stable up to twelve months from date of receipt; working aliquots with carrier protein stable ~3 months) and avoid repeated freeze/thaw cycles. Product origin is the USA. Kingfisher Biotech products are supplied for research applications and are not intended for medicinal, diagnostic, or therapeutic use. It is commonly used in immunology and cell-biology research to study interferon signaling and antiviral responses, induction of interferon-stimulated genes and antiviral states, and for antiviral/cytopathic-effect assays; typical experimental uses include cell-culture stimulation and dosing studies, ELISA and neutralization assays, receptor-binding studies, flow-cytometry controls, and antibody generation/validation.

Amino Acid Sequence	CHLPHTHSLA NRRVLMLLQQ LRRVSPSSCL QDRNDFEFLQ EALGGSQLQK AQAISVLHEV TQHTFQLFST EGSPATWDKS LLDKLRAALD QQLTDLQACL TQEEGLRGAP LLKEDSSLAV RKYFHRLTLY LQEKRHSPCA WEVVRAEVMR AFSSSTNLQE SFRRKD (166)
Endotoxin	Naturally endotoxin-free

Storage Conditions	-20°C
Molecular Weight	19.0 kDa
Purity	>98% as visualized by SDS-PAGE analysis.
Country Of Origin	USA

Bovine Interferon-Alpha A (IFN-αA) is a type I interferon subtype produced primarily by plasmacytoid dendritic cells and virus-infected cells in cattle (Bos taurus), where it plays a central role in early innate antiviral defense. Upon binding to the type I interferon receptor complex (IFNAR1/IFNAR2), bovine IFN-αA activates the JAK/STAT signaling pathway, leading to induction of interferon-stimulated genes (ISGs) that inhibit viral replication, enhance antigen presentation, and promote activation of natural killer (NK) cells and adaptive immune responses. In bovine health, IFN-αA is critically involved in host responses to economically significant viral infections such as bovine viral diarrhea virus (BVDV), bovine herpesvirus-1 (IBR), bovine respiratory syncytial virus (BRSV), foot-and-mouth disease virus (FMDV), and other components of the bovine respiratory disease (BRD) complex. Altered or suppressed IFN-α responses-such as immune evasion by BVDV-can contribute to viral persistence and increased disease severity. As both a biomarker and functional mediator of antiviral immunity, bovine IFN-αA is important in vaccine evaluation, antiviral strategy development, and immunopathogenesis studies. In translational research, characterization of bovine IFN-αA supports comparative studies of type I interferon biology, viral immune evasion mechanisms, and large-animal models of respiratory and systemic viral infection relevant to both veterinary and human medicine.

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