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CartBovine IL-8 (CXCL8) (Yeast-derived Recombinant Protein) - 5 micrograms
Bulk quantities of Bovine IL-8 protein are available.
Please contact us for pricing.
Bovine IL-8 Specifications
Molecular Weight (calculated) - 9.1kDa
Amino Acid Sequence - AVLSRMSTEL RCQCIKTHST PFHPKFIKEL RVIESGPHCE NSEIIVKLTN GNEVCLNPKE KWVQKVVQVF VKRAEKQDP (79)
Gene ID - 280828
Homology Across Species
Bos taurus (cattle) IL-8 – 100%
Bos indicus (zebu) IL-8 – 100%
Bison bison bison (bison) IL-8 – 99%
Bubalus bubalis (water buffalo) IL-8 – 99%
Cervus elaphus (red deer) IL-8 – 97%
Pantholops hodgsonii (chiru) IL-8 – 96%
Ovis aries (sheep) IL-8 – 95%
More - https://blast.ncbi.nlm.nih.gov/
Endotoxin - Naturally endotoxin-free
Applications
Cell Culture, ELISA Standard, Western Blot Control
IL-8 Background
Interleukin-8 (IL-8), also known as CXCL8, is a CXC family member chemokine produced by macrophages and other cell types such as epithelial cells. There have been 17 different CXC chemokines described in mammals, that are subdivided into two categories, those with a specific amino acid sequence (or motif) of glutamic acid-leucine-arginine (or ELR for short) immediately before the first cysteine of the CXC motif (ELR-positive), and those without an ELR motif (ELR-negative). ELR-positive CXC chemokines such as IL-8 specifically induce the migration of neutrophils, and interact with chemokine receptors CXCR1 and CXCR2.
Alternate Names - CXCL8, chemokine (C-X-C motif) ligand 8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, IL8, C-X-C motif chemokine ligand 8, Interleukin-8
Host Factors Determine the Evolution of Infection With Staphylococcus Aureus to Gangrenous Mastitis in Goats.
Rainard P, Gitton C, Chaumeil T, Fassier T, Huau C, Riou M, Tosser-Klopp G, Krupova Z, Chaize A, Gilbert FB, Rupp R, Martin P.
Vet Res. 2018 Jul 25;49(1):72. doi: 10.1186/s13567-018-0564-4.
Applications: Measurement of goat IL-8 in milk by ELISA
Abstract
Staphylococcus aureus is the major cause of very severe mastitis of dairy goats. The initial objective of our study was to fine-tune an experimental model of infection of the goat mammary gland with two strains of S. aureus and two lines of goats (low and high somatic cell score lines). Following the challenge, the 10 infected goats divided in two clear-cut severity groups, independently of the S. aureus strain and the goat line. Five goats developed very severe mastitis (of which four were gangrenous) characterized by uncontrolled infection (UI group), whereas the other five kept the infection under control (CI group). The outcome of the infection was determined by 18 h post-infection (hpi), as heralded by the bacterial milk concentration at 18 hpi: more than 107/mL in the UI group, about 106/mL in the CI group. Leukocyte recruitment and composition did not differ between the groups, but the phagocytic killing at 18 hpi efficiency did. Contributing factors involved milk concentrations of α-toxin and LukMF' leukotoxin, but not early expression of the genes encoding the pentraxin PTX3, the cytokines IL-1α and IL-1β, and the chemokines IL-8 and CCL5. Concentrations of TNF-α, IFN-γ, IL-17A, and IL-22 rose sharply in the milk of UI goats when infection was out of control. The results indicate that defenses mobilized by the mammary gland at an early stage of infection were essential to prevent staphylococci from reaching critical concentrations. Staphylococcal exotoxin production appeared to be a consequent event inducing the evolution to gangrenous mastitis.
Possible role of interferon tau on the bovine corpus luteum and neutrophils during the early pregnancy.
Shirasuna K, Matsumoto H, Matsuyama S, Kimura K, Bollwein H, Miyamoto A.
Reproduction. 2015 Sep;150(3):217-25. doi: 10.1530/REP-15-0085. Epub 2015 Jun 15.
Applications: Stimulation of chemotaxis of PMS in culture
Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation.
Verbeke J, Boulougouris X, Rogiers C, Burvenich C, Peelman L, De Spiegeleer B, De Vliegher S.
BMC Vet Res. 2015 May 6;11:104. doi: 10.1186/s12917-015-0418-5.
Applications: Stimulation of bovine neutrophils in cell culture.
Abstract
BACKGROUND:
Associations between polymorphisms in the bovine CXCR1 gene, encoding the chemokine (C-X-C motif) receptor 1 (IL8RA), and neutrophil traits and mastitis have been described. In the present study, blood neutrophils were isolated from 20 early lactating heifers with different CXCR1 genotype at position 735 or 980. The cells were incubated with different concentrations of recombinant bovine IL-8 (rbIL-8) for 2 or 6 h and stimulated with phorbol 12-myristate 13-acetate (PMA) or opsonized zymosan particles (OZP). Potential association between CXCR1 genotype and production of reactive oxygen species (ROS) was studied.
RESULTS:
Although on single nucleotide polymorphisms (SNPs) may potentially affect CXCR1 function, SNPs c.735C > G and c.980A > G showed no association with ROS production with or without incubation of rbIL-8. Neutrophils incubated with rbIL-8 for 2 or 6 h showed higher PMA- and lower OZP-induced ROS production compared to control without rbIL-8.
CONCLUSIONS:
In the present study no association could be detected between superoxide production by isolated bovine neutrophils during early lactation and CXCR1 gene polymorphism. IL-8 showed to possess inhibitory effects on ROS generation in bovine neutrophils.
Enzyme linked immunosorbent assay for quantification of bovine interleukin-8 to study infection and immunity in the female genital tract.
Cronin JG, Hodges R, Pedersen S, Sheldon IM.
Am J Reprod Immunol. 2015 Apr;73(4):372-82. doi: 10.1111/aji.12344. Epub 2014 Nov 27.
Applications: Bovine IL-8 protein was used as an ELISA standard. Bovine IL-10 and CCL2 were used to check the ELISA for specificity.
Strain-specific pathogenicity of putative host-adapted and nonadapted strains of Streptococcus uberis in dairy cattle.
Tassi R, McNeilly TN, Fitzpatrick JL, Fontaine MC, Reddick D, Ramage C, Lutton M, Schukken YH, Zadoks RN.
J Dairy Sci. 2013 Jun 12.
Applications: Measurement of Bovine IL-17A in Milk using the Bovine IL-17A VetSet. Use of Bovine IL-8 Protein as an ELISA standard.
| Streptococcus uberis is an important cause of intramammary infection in dairy cattle. Strains of Strep. uberis appear to differ in their ability to cause disease based on previous epidemiological studies. We explored the pathogenicity of 2 strains of Strep. uberis, where one strain represented a putatively host-adapted type based on its ability to cause persistent infection and to spread from cow to cow in a lactating herd. This type was part of a clonal complex that is commonly associated with bovine mastitis. The other strain, which was isolated from a transient infection in a single animal in the same herd and did not belong to any known clonal complex, was selected as putatively nonadapted type. Cows (6 per strain) were experimentally challenged in a single hind quarter and the adjacent hind quarter was used as mock challenged control quarter. Both strains showed an equal ability to grow in the milk of challenge animals in vitro. All cows that were challenged with the putatively host-adapted strain developed clinical signs of mastitis, including fever and milk yield depression as well as elevated somatic cell count due to influx of polymorphonuclear leucocytes and lymphocytes. The cytokine response followed a specific order, with an increase in IL-1β, IL-6, and IL-8 levels at the time of first SCC elevation, followed by an increase in IL-10, IL-12p40, and tumor necrosis factor-α levels approximately 6h later. In 4 of 6 animals, IL-17A was detected in milk between 57 and 168 h postchallenge. The increase in IL-17A levels coincided with inversion of the prechallenge CD4(+)-to-CD8(+) T lymphocyte ratio, which was observed from 96 h postchallenge. This was followed by normalization of the CD4(+)-to-CD8(+) ratio due to continued increase of the CD8(+) concentration up to 312 h postchallenge. Spontaneous resolution of infection was observed in 5 animals and coincided with a measurable IL-17A response in 4 animals, suggesting that IL-17 may be involved in the resolution of intramammary infection. With the exception of minor elevation of IL-8 levels, no clinical, cytological, or immunological response was detected in quarters challenged with the nonadapted strain. The observed strain-specific pathogenicity was consistent across animals, implying that it is determined by pathogen factors rather than host factors. |
Intra-Amniotic Administration of E coli Lipopolysaccharides Causes Sustained Inflammation of the Fetal Skin in Sheep.
Zhang L, Saito M, Jobe A, Kallapur SG, Newnham JP, Cox T, Kramer B, Yang H, Kemp MW.
Reprod Sci. 2012 May 17.
Applications: ELISA Standard
Identification of single nucleotide polymorphisms in the bovine Toll-like receptor 1 gene and association with health traits in cattle.
Russell CD, Widdison S, Leigh JA, Coffey TJ.
Vet Res. 2012 Mar 14;43(1):17.
Applications: ELISA Standard
Rapid accumulation of polymorphonuclear neutrophils in the Corpus luteum during prostaglandin F(2α)-induced luteolysis in the cow.
Shirasuna K, Jiemtaweeboon S, Raddatz S, Nitta A, Schuberth HJ, Bollwein H, Shimizu T, Miyamoto A.
PLoS One. 2012;7(1):e29054.
Applications: Stimulation of Bovine Neutrophils
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