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CartCanine IL-6 (Yeast-derived Recombinant Protein) - 5 micrograms
Bulk quantities of Canine IL-6 protein are available.
Please contact us for pricing.
Canine IL-6 Specifications
Molecular Weight (calculated) - 21.0kDa
Amino Acid Sequence - FPTPGPLAGD SKDDATSNSL PLTSANKVEE LIKYILGKIS ALRKEMCDKF NKCEDSKEAL AENNLHLPKL EGKDGCFQSG FNQETCLTRI TTGLVEFQLH LNILQNNYEG DKENVKSVHM STKILVQMLK SKVKNQDEVT TPDPTTDASL QAILQSQDEW LKHTTIHLIL RSLEDFLQFS LRAVRIM (187)
Gene ID - 403985
Homology Across Species
Canis lupus familiaris (dog) IL-6 – 100%
Canis lupus dingo (dingo) IL-6 – 100%
Vulpes lagopus (Arctic fox) IL-6 – 97%
Vulpes vulpes (red fox) IL-6 – 97%
More - https://blast.ncbi.nlm.nih.gov/
Endotoxin - Naturally endotoxin-free
Applications
Cell Culture, ELISA Standard, Western Blot Control
IL-6 Background
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also produced from muscle, and is elevated in response to muscle contraction. It is significantly elevated with exercise, and precedes the appearance of other cytokines in the circulation. Osteoblasts secrete IL-6 to stimulate osteoclast formation. Smooth muscle cells in the tunica media of many blood vessels also produce IL-6 as a pro-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1ra and IL-10.
Alternate Names - IL6, BSF2, HGF, HSF, IFNB2, IL-6, BSF-2, CDF, IFN-beta-2, interleukin 6
Elevated circulating Th2 but not group 2 innate lymphoid cell responses characterize canine atopic dermatitis.
Früh SP, Saikia M, Eule J, Mazulis CA, Miller JE, Cowulich JM, Oyesola OO, Webb LM, Peng SA, Cubitt RL, Danko CG, Miller WH, Tait Wojno ED.
Vet Immunol Immunopathol. 2020 Jan 24;221:110015. doi: 10.1016/j.vetimm.2020.110015. [Epub ahead of print]
Applications: In vitro culture of CD4+ T cells and CD25+ ILCs
Th17 Pathway As a Target for Multipotent Stromal Cell Therapy in Dogs: Implications for Translational Research.
Kol A, Walker NJ, Nordstrom M, Borjesson DL.
PLoS One. 2016 Feb 12;11(2):e0148568. doi: 10.1371/journal.pone.0148568. eCollection 2016.
Applications: Canine IL-6 and IL-1 beta proteins were use to stimulate T cells in culture.
Abstract
Detrimental Th17 driven inflammatory and autoimmune disease such as Crohn's disease, graft versus host disease and multiple sclerosis remain a significant cause of morbidity and mortality worldwide. Multipotent stromal/stem cell (MSC) inhibit Th17 polarization and activation in vitro and in rodent models. As such, MSC based therapeutic approaches are being investigated as novel therapeutic approaches to treat Th17 driven diseases in humans. The significance of naturally occurring diseases in dogs is increasingly recognized as a realistic platform to conduct pre-clinical testing of novel therapeutics. Full characterization of Th17 cells in dogs has not been completed. We have developed and validated a flow-cytometric method to detect Th17 cells in canine blood. We further demonstrate that Th17 and other IL17 producing cells are present in tissues of dogs with naturally occurring chronic inflammatory diseases. Finally, we have determined the kinetics of a canine specific Th17 polarization in vitro and demonstrate that canine MSC inhibit Th17 polarization in vitro, in a PGE2 independent mechanism. Our findings provide fundamental research tools and suggest that naturally occurring diseases in dogs, such as inflammatory bowel disease, may be harnessed to translate novel MSC based therapeutic strategies that target the Th17 pathway.
Interleukin-1β, tumour necrosis factor-α and lipopolysaccharide induce C-type natriuretic peptide from canine aortic endothelial cells.
Osterbur K, Yu DH, Declue AE.
Res Vet Sci. 2012 Nov 8. doi:pii: S0034-5288(12)00305-0. 10.1016/j.rvsc.2012.10.002.
Applications: Stimulation of canine aortic endothelial cells
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