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Chicken IL-4 (Yeast-derived Recombinant Protein) - 5 micrograms

RP0110C-005
$150.00
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Bulk quantities of Chicken IL-4 protein are available.
Please contact us for pricing.

Chicken IL-4 Specifications

Molecular Weight (calculated) - 12.4kDa

Amino Acid Sequence - LQLSVPLMES IRIVNDIQGE VSCVKMNVTD IFADNKTNNK TELLCKASTI VWESQHCHKN LQGLFLNMRQ LLNASSTSLK APCPTAAGNT TSMEKFLADL RTFFHQLAKN K (111)

Gene ID - 416330

Homology Across Species
Gallus gallus (chicken) IL-4 - 100%
Gallus lafayetii (Ceylon junglefowl) IL-4 - 100%
Gallus sonneratii (gray junglefowl) IL-4 - 100%
More - https://blast.ncbi.nlm.nih.gov/

Endotoxin - Naturally endotoxin-free

Applications

Cell Culture, ELISA Standard, Western Blot Control

IL-4 Background

Interleukin-4 (IL-4) induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4. IL-4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B-cell class switching to IgE, and up-regulates MHC class II production. Overproduction of IL-4 is associated with allergies.

Alternate Names - IL4, BCGF-1, BCGF1, BSF-1, BSF1, IL-4, interleukin 4

Chicken IL-4 (Yeast-derived Recombinant Protein) - 5 micrograms
Catalog No.:
RP0110C-005
Quantity:
5 ug
Source:
The Chicken IL-4 recoombinant protein was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
The Chicken IL-4 recombinant protein has a predicted molecular weight of 12.4 kDa.
Protein Sequence:
LQLSVPLMES IRIVNDIQGE VSCVKMNVTD IFADNKTNNK TELLCKASTI VWESQHCHKN LQGLFLNMRQ LLNASSTSLK APCPTAAGNT TSMEKFLADL RTFFHQLAKN K (111)
Country of Origin:
USA
Applications:
The Chicken IL-4 endotoxin-free recombinant protein can be used in cell culture, as an IL-4 ELISA Standard, and as a Western Blot Control.

32429204

Pustulan Activates Chicken Bone Marrow-Derived Dendritic Cells In Vitro and Promotes Ex Vivo CD4+ T Cell Recall Response to Infectious Bronchitis Virus.

Renu S, Feliciano-Ruiz N, Lu F, Ghimire S, Han Y, Schrock J, Dhakal S, Patil V, Krakowka S, HogenEsch H, Renukaradhya GJ.

Vaccines (Basel). 2020 May 15;8(2):E226. doi: 10.3390/vaccines8020226.

Applications: IL-4 and GM-CSF were used to generate dendritic cells from chicken bone marrow cells in culture.

Abstract

Infectious bronchitis virus (IBV) is a highly contagious avian coronavirus. IBV causes substantial worldwide economic losses in the poultry industry. Vaccination with live-attenuated viral vaccines, therefore, are of critical importance. Live-attenuated viral vaccines, however, exhibit the potential for reversion to virulence and recombination with virulent field strains. Therefore, alternatives such as subunit vaccines are needed together with the identification of suitable adjuvants, as subunit vaccines are less immunogenic than live-attenuated vaccines. Several glycan-based adjuvants directly targeting mammalian C-type lectin receptors were assessed in vitro using chicken bone marrow-derived dendritic cells (BM-DCs). The β-1-6-glucan, pustulan, induced an up-regulation of MHC class II (MHCII) cell surface expression, potentiated a strong proinflammatory cytokine response, and increased endocytosis in a cation-dependent manner. Ex vivo co-culture of peripheral blood monocytes from IBV-immunised chickens, and BM-DCs pulsed with pustulan-adjuvanted recombinant IBV N protein (rN), induced a strong recall response. Pustulan-adjuvanted rN induced a significantly higher CD4+ blast percentage compared to either rN, pustulan or media. However, the CD8+ and TCRγδ+ blast percentage were significantly lower with pustulan-adjuvanted rN compared to pustulan or media. Thus, pustulan enhanced the efficacy of MHCII antigen presentation, but apparently not the cross-presentation on MHCI. In conclusion, we found an immunopotentiating effect of pustulan in vitro using chicken BM-DCs. Thus, future in vivo studies might show pustulan as a promising glycan-based adjuvant for use in the poultry industry to contain the spread of coronaviridiae as well as of other avian viral pathogens.


27238770

Distinct functional responses to stressors of bone marrow derived dendritic cells from diverse inbred chicken lines.

Van Goor A, Slawinska A, Schmidt CJ, Lamont SJ.

Dev Comp Immunol. 2016 Oct;63:96-110. doi: 10.1016/j.dci.2016.05.016. Epub 2016 May 27.

Applications: Generation of bone marrow derived dendritic cells


26813292

Interaction of a Live Attenuated Salmonella Gallinarum Vaccine Candidate With Chicken Bone Marrow-Derived Dendritic Cells.

Kamble NM, Jawale CV, Lee JH.

Avian Pathol. 2016;45(2):235-43. doi: 10.1080/03079457.2016.1144919.

Applications: Stimulation of chicken bone marrow-derived dendritic cells (chBM-DCs) with chicken GM-CSF and IL-4 in culture.

Abstract

Salmonella enterica serovar Gallinarum (SG) is a Gram-negative intracellular host-adapted pathogen that causes fowl typhoid. Attenuated strains of SG are proven and widely used vaccine candidates because of advantages like induction of strong humoral and cell-mediated immune responses. In the present study, we investigated the interaction of chicken bone marrow-derived dendritic cells (chBM-DCs) with an attenuated SG (JOL1355) strain that secretes a heat-labile enterotoxin B subunit protein previously shown to successfully vaccinate chickens. ChBM-DCs were isolated and cultured in the presence of recombinant chicken GM-CSF and IL-4 cytokines. The chBM-DCs were infected with JOL1355 at an multiplicity of infection of 10. JOL1355 was able to invade dendritic cells (DCs); however, the survival of JOL1355 in DCs decreased over time. At 24 h post infection, IL-6, IL-10 and IFN-γ transcript levels were significantly increased in JOL1355-infected DCs compared to non-stimulated DCs. Flow cytometry analysis showed an increased proportion of cells producing CD40, CD80, and MHC class II in the JOL1355-infected cultures compared to the non-stimulated control. In addition, JOL1355-stimulated chBM-DCs could induce significant expression of IL-2 in co-culture with autologous CD4+ T cells. Based on these results, we conclude that chBM-DCs are capable of internalizing the live attenuated SG vaccine candidate and the infected chBM-DCs show signs of maturation as evidenced by the upregulated expression of costimulatory molecules and cytokines.

 


22197009

Interleukin 4 increases CCR9 expression and homing of lymphocytes to gut-associated lymphoid tissue in chickens.

Annamalai T, Selvaraj RK.

Vet Immunol Immunopathol. 2012 Jan 15;145(1-2):257-63.

Applications: Cell Culture Stimulation

Abstract
The effects of in vitro and in vivo IL-4 supplementation on thymocyte and splenocyte CCR9 mRNA amount and migration were studied. Thymocytes, splenocytes, splenocytes+thymocytes (2:1), and splenocytes+bursocyte cells (2:1) were supplemented with either 0 or 5 ng/ml IL-4 for 5d. CCR9 mRNA was undetectable in all experimental groups supplemented with 0 ng/ml IL-4. IL-4 treatment (5 ng/ml) upregulated (P=0.01) CCR9 mRNA only in the splenocyte+thymocyte cell culture. IL-4-mediated CCR9 mRNA induction in the splenocyte+thymocyte cell culture was dependent on the in vitro dose of IL-4 supplementation. IL-4-treated splenocyte+thymocyte cells when injected in vivo preferentially migrated to cecal tonsils. In vivo supplementation of IL-4 was achieved through in ovo injection of recombinant chicken IL-4 plasmid. Cecal tonsils in chicks hatched from IL-4-plasmid-injected eggs weighed more, had a higher amount of CCR9 mRNA, and had a higher percentage of CD8(+) cells than cecal tonsils from chicks hatched from PBS-injected eggs. It could be concluded that IL-4 induces CCR9 mRNA in thymocytes and splenocytes and directs the migration of cells to gut-associated lymphoid tissue.


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