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Rat APRIL (TNFSF13) (Yeast-derived Recombinant Protein) - 5 micrograms

RP0383R-005
$150.00
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Bulk quantities of Rat APRIL protein are available.
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Rat APRIL Specifications

Molecular Weight (calculated) - 16.4kDa

Amino Acid Sequence - AVLTQKHKKK QSVLHLVPIN ITSKADSDMT EVMWQPALRR GRGLEAQGDT VRVRDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIKSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL (146)

Gene ID - 287437

Homology Across Species
Rattus norvegicus (Norway rat) APRIL – 100%
Rattus rattus (black rat) APRIL – 99%
More - https://blast.ncbi.nlm.nih.gov/

Endotoxin - Naturally endotoxin-free

Applications

Cell Culture, ELISA Standard, Western Blot Control

APRIL Background

Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a member of the tumor necrosis factor ligand (TNF) ligand family. Nineteen cytokines have been identified as part of the TNF family on the basis of sequence, functional, and structural similarities. Family members include TNF beta (TNFSF1), TNF alpha (TNFSF2), Lymphotoxin beta (TNFSF3), OX40 Ligand (TNFSF4), CD40 Ligand (TNFSF5), Fas Ligand (TNFSF6), CD27 Ligand (TNFSF7), CD30 Ligand (TNFSF8), 4-1BB Ligand (TNFSF9), TRAIL (TNFSF10), TRANCE/RANKL (TNFSF11), TWEAK (TNFSF12), APRIL(TNFSF13), BAFF (TNFSF13B), LIGHT (TNFSF14), TL1A/VEGI (TNFSF15), and GITR Ligand (TNFSF18). APRIL/TNFSF13 is expressed by macrophages and dendritic cells. APRIL/TNFSF13 has been shown to play a role in protecting cells from undergoing apoptosis and promoting B cell development.

Alternate Names - TNFSF13, APRIL, CD256, TALL-2, TALL2, TRDL-1, ZTNF2, UNQ383/PRO715, TNLG7B, tumor necrosis factor superfamily member 13, TNF superfamily member 13

Rat APRIL (TNFSF13) (Yeast-derived Recombinant Protein) - 5 micrograms
Catalog No.:
RP0383R-005
Quantity:
5 ug
Source:
Rat APRIL was produced in yeast and therefore does not have endotoxin, is naturally folded, and post-translationally modified.
MW:
Rat APRIL has a predicted molecular weight of 16.4 kDa.
Protein Sequence:
AVLTQKHKKK QSVLHLVPIN ITSKADSDMT EVMWQPALRR GRGLEAQGDT VRVRDTGIYL LYSQVLFHDV TFTMGQVVSR EGQGRRETLF RCIKSMPSDP DRAYNSCYSA GVFHLHQGDI ITVKIPRANA KLSLSPHGTF LGFVKL (146)
Alias:
TNFSF13
Country of Origin:
USA
Applications:
The rat APRIL endotoxin-free recombinant protein can be used in cell culture, as an ELISA Standard, and as a Western Blot Control.

25466610

APRIL promotes proliferation, secretion and invasion of fibroblast-like synoviocyte from rats with adjuvant induced arthritis.

Chang, Y; Jia, X; Sun, X; Xu, S; Wu, Y; Zhang, L; Wei, W

Mol Immunol. 2015 Mar;64(1):90-8. doi: 10.1016/j.molimm.2014.11.002. Epub 2014 Nov 23.

Applications: Measurement of Rat APRIL in synovium homogenate samples.

Abstract
Fibroblast-like synoviocyte (FLS) is the ultimate effectual cells in the pathogenesis of rheumatoid arthritis (RA). The current study was undertaken to investigate whether a proliferation-inducing ligand (APRIL) mediates the function of FLS in an animal model of RA. Rat adjuvant-induced arthritis (AA) was induced by intradermal injection of 0.1 ml complete Freund's adjuvant. Synovium APRIL expression was detected by immunohistochemical analysis. The level of APRIL and matrix metalloproteinase (MMP)-9 were assayed by enzyme-linked immunosorbent assay. The expression of APRIL and its receptors (TACI, BCMA and BAFF-R) were assessed by immunofluorescence staining, flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR. The effects of APRIL on the function of FLS were investigated by MTT, Quantibody Rat Inflammation Array 1 and transwell assays, respectively. A higher concentration of APRIL was detected in AA synovium homogenate compared with normal group. AA FLS expressed APRIL, TACI, BAFF-R and BCMA at the mRNA levels, whereas only APRIL and BCMA were confirmed to be expressed on membrane by flow cytometry. APRIL stimulated AA FLS proliferation, migration and invasion and the secretion of proinflammatory factors. In addition, FLS cocultured with APRIL-treated B cells or T cells had a significantly greater proliferation than FLS cultured alone. Neutralization of APRIL by the TACI-Ig fusion protein attenuated these stimulating effects of APRIL on FLS. Our data indicate that APRIL may act as an important mediator for facilitating the function of FLS. Blockade of APRIL thus may be a valuable adjunct in the treatment of RA.


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